2. Academic Validation
  3. Bright photoactivatable fluorophores for single-molecule imaging

Bright photoactivatable fluorophores for single-molecule imaging

  • Nat Methods. 2016 Dec;13(12):985-988. doi: 10.1038/nmeth.4034.
Jonathan B Grimm 1,  Brian P English 1,  Heejun Choi 1,  Anand K Muthusamy 1,  Brian P Mehl 1,  Peng Dong 1,  Timothy A Brown 1,  Jennifer Lippincott-Schwartz 1,  Zhe Liu 1,  Timothée Lionnet 1,  Luke D Lavis 1
Affiliations

Affiliation

  • 1 Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA.
PMID: 27776112 DOI: 10.1038/nmeth.4034
Abstract

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

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