2. Academic Validation
  3. Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay

Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay

  • Food Chem. 2017 Apr 15;221:1813-1821. doi: 10.1016/j.foodchem.2016.10.099.
Zhonghui Li 1,  Zhoumin Li 2,  Danke Xu 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: lizhonghui0106@gmail.com.
  • 2 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China; School of Chemistry and Biological Science, Nanjing University Jingling College, Nanjing 210089, China. Electronic address: lizhoumin@126.com.
  • 3 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: xudanke@nju.edu.cn.
PMID: 27979167 DOI: 10.1016/j.foodchem.2016.10.099
Abstract

A visualized microarray sensing technique has been developed and applied to the screening of honey samples for residues of banned nitrofuran Antibiotics. Using a multiplexed approach, metabolites of four main nitrofuran Antibiotics can be detected simultaneously. Individual antigens were spotted onto 96-well plates. An indirective competitive assay format, with visualized signal response, was employed. An extraction method, based on derivatization with 2-nitrobenzaldehyde and partition into ethyl acetate, was used for screening. The limits of detection were 0.10, 0.04, 0.04, and 0.10ngg-1 for 3-amino-5-morpholino-2-oxazolidone (AMOZ), 3-amino-2-oxazolidinone (AOZ), semicarbazide (SEM), and 1-aminohydantoin (AHD), respectively. The recovery rate ranged from 78% to 93% for the four targets. In addition, this method was easy to operate with low detection cost and fast speed. This microarray method possesses the potential to be a fit-for-purpose screening technique in the arena of food safety monitoring.

Keywords

2-NP-AHD (PubChem CID: 9690702); 2-NP-AMOZ (PubChem CID: 53393538); 2-NP-AOZ (PubChem CID: 209292); 2-NP-SEM (PubChem CID: 6870110); AHD (PubChem CID: 72823); AMOZ (PubChem CID: 3016406); AOZ (PubChem CID: 65725); Chloroauric acid (PubChem CID: 28133); Nitrofuran metabolites; SEM (PubChem CID: 5196); Silver nanoparticles; Silver nitrate (PubChem CID: 24470); Visualized microarray assay.

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