2. Academic Validation
  3. Development of a broad-specificity antibody-based immunoassay for triazines in ginger and the quantitative structure-activity relationship study of cross-reactive molecules by molecular modeling

Development of a broad-specificity antibody-based immunoassay for triazines in ginger and the quantitative structure-activity relationship study of cross-reactive molecules by molecular modeling

  • Anal Chim Acta. 2018 Jul 5:1012:90-99. doi: 10.1016/j.aca.2018.01.042.
Congmin Liu 1,  Xiaowen Dou 1,  Lei Zhang 1,  Weijun Kong 1,  Liu Wu 2,  Yaping Duan 2,  Meihua Yang 3
Affiliations

Affiliations

  • 1 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China.
  • 2 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China; College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun, 130118, China.
  • 3 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China. Electronic address: yangmeihua15@hotmail.com.
PMID: 29475478 DOI: 10.1016/j.aca.2018.01.042
Abstract

In the present study, molecular modeling and principle component analysis (PCA) were used to select appropriate haptens for group detection of triazine herbicides. Four new structures together with three reported triazine derivatives were chosen for the screening of immunizing and coating haptens. A total of 31 triazines coupled with a 3D-QSAR methodology were employed to investigate the relationship between antigen-antibody recognition and molecular structures, the results of which revealed that the Antibodies may recognize triazines from the side of molecules with the distinguishing atom and a steric volume matching with the spatial structure of Antibodies. Finally, a broad-specificity heterologous immunoassay was developed for determining 10 triazine herbicides in ginger, where the detection limits were 2.5-15.1 μg kg-1 and recoveries were 67.9-102.6%. This study may broaden insight into triazine-antibody interactions and benefit designing novel performance-enhanced Antibodies. The developed immunoassay can be further used for triazine detection in other complicated matrices.

Keywords

Antibody; Broad-specificity immunoassay; Molecular modeling; Quantitative structure-activity relationship; Triazine herbicides.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z