1. Academic Validation
  2. Physalin B induces cell cycle arrest and triggers apoptosis in breast cancer cells through modulating p53-dependent apoptotic pathway

Physalin B induces cell cycle arrest and triggers apoptosis in breast cancer cells through modulating p53-dependent apoptotic pathway

  • Biomed Pharmacother. 2018 May;101:334-341. doi: 10.1016/j.biopha.2018.02.094.
Anqi Wang 1 Shengpeng Wang 1 Fayang Zhou 1 Peng Li 1 Yitao Wang 1 Lishe Gan 2 Ligen Lin 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, PR China.
  • 2 College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: lsgan@zju.edu.cn.
  • 3 State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, PR China. Electronic address: ligenl@umac.mo.
Abstract

Physalin B (PB), one of the major active steroidal constituents of Cape gooseberry (Physalis alkekengi L.), possesses a wide spectrum of biological activities. Although the Anticancer activity of PB was reported in previous studies, the underlying mechanisms are still not well stated. In this study, the Anticancer effect and the underlying mechanisms of PB were investigated in breast Cancer cells. PB significantly reduced the viability of three human breast Cancer cell lines, MCF-7, MDA-MB-231 and T-47D, in a concentration- and time-dependent manner. PB induced cell cycle arrest at G2/M phase and promoted cleavage of PARP (poly (ADP-ribose) polymerase), caspases 3, Caspase 7 and Caspase 9 to stimulate cell Apoptosis. Further studies showed that PB induced breast Cancer cells Apoptosis in a p53-dependent manner in MCF-7 cells. PB also suppressed the phosphorylation of Akt (protein kinase B) and PI3K (phosphoinositide 3-kinase), and increased the phosphorylation of GSK-3β (glycogen synthase kinase 3β). Taken together, our results indicated that PB might serve as a potential therapeutic agent for breast Cancer.

Keywords

Akt; Apoptosis; Breast cancer; Cell cycle; Physalin B; p53.

Figures
Products
我们的 Cookie 政策

我们使用 Cookies 和类似技术以提高网站的性能和提升您的浏览体验,部分功能也使用 Cookies 帮助我们更好地理解您的需求,为您提供相关的服务。 如果您有任何关于我们如何处理您个人信息的疑问,请阅读我们的《隐私声明》