2. Academic Validation
  3. Methionine-Containing Rhabdopeptide/Xenortide-like Peptides from Heterologous Expression of the Biosynthetic Gene Cluster kj12ABC in Escherichia coli

Methionine-Containing Rhabdopeptide/Xenortide-like Peptides from Heterologous Expression of the Biosynthetic Gene Cluster kj12ABC in Escherichia coli

  • J Nat Prod. 2018 Oct 26;81(10):2292-2295. doi: 10.1021/acs.jnatprod.8b00425.
Lei Zhao 1 2,  Xiaofeng Cai 1,  Marcel Kaiser 3 4,  Helge B Bode 1 5
Affiliations

Affiliations

  • 1 Molekulare Biotechnologie, Fachbereich Biowissenschaften , Goethe Universität Frankfurt , 60438 Frankfurt am Main , Germany.
  • 2 Institute of Botany , Jiangsu Province and Chinese Academy of Sciences , 210014 Nanjing , China.
  • 3 Parasite Chemotherapy , Swiss Tropical and Public Health Institute , 4051 Basel , Switzerland.
  • 4 University of Basel , 4003 Basel , Switzerland.
  • 5 Buchmann Institute for Molecular Life Sciences (BMLS) , Goethe Universität Frankfurt , 60438 Frankfurt am Main , Germany.
PMID: 30302998 DOI: 10.1021/acs.jnatprod.8b00425
Abstract

Seven new methionine-containing rhabdopeptide/xenortide-like Peptides (1-7) were identified from Escherichia coli expressing the rhabdopeptide/xenortide-like peptide biosynthetic gene cluster kj12ABC from Xenorhabdus KJ12.1. Their structures were elucidated by detailed HPLC-HR-MS/MS analysis and confirmed by chemical synthesis. Bioactivity tests of these first rhabdopeptide/xenortide-like peptide derivatives (2-7) showing methionine building blocks compared to the usually found derivatives containing exclusively hydrophobic Amino acids such as valine, leucine, or phenylalanine revealed good activities of 2-7 against protozoan parasites and no cytotoxicity against mammalian L6 cells.

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