2. Academic Validation
  3. Esculentoside H inhibits colon cancer cell migration and growth through suppression of MMP-9 gene expression via NF-kB signaling pathway

Esculentoside H inhibits colon cancer cell migration and growth through suppression of MMP-9 gene expression via NF-kB signaling pathway

  • J Cell Biochem. 2019 Jun;120(6):9810-9819. doi: 10.1002/jcb.28261.
Sun-Hyung Ha 1,  Kyung-Min Kwon 1,  Jun-Young Park 1,  Fukushi Abekura 1,  Young-Choon Lee 2,  Tae-Wook Chung 3,  Ki-Tae Ha 3,  Hyeun Wook Chang 4,  Seung-Hak Cho 5,  Jong-Suk Kim 6,  Cheorl-Ho Kim 1
Affiliations

Affiliations

  • 1 Molecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Chunchun-Dong, Jangan-Gu, Suwon, Kyunggi-Do, Korea.
  • 2 Department of Biological Sciences, Faculty of Medicinal Biotechnology, Dong-A University, Busan, Republic of Korea.
  • 3 Division of Applied Medicine, Healthy Aging Korean Medical Research Center, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
  • 4 Department of Pharmacy, Yeungnam University, Daehak-Ro, Gyeongsan, Gyeongbuk, Republic of Korea.
  • 5 Division of Bacterial Disease Research, Center for Infectious Disease Research, Korea National Institute of Health, Cheongju, Korea.
  • 6 Department of Biochemistry, Institute for Medical Sciences, Chonbuk National University Medical School, Baekje-daero, Deokjin-gu, Jeonju, Korea.
PMID: 30525244 DOI: 10.1002/jcb.28261
Abstract

A water-soluble saponin, Esculentoside H (EsH), 3-O-(O-β-d-glucopyranosyl-(1→4)-β-d-xylopyranosyl)-28-β-d-glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta. EsH is known to be an Anticancer compound, having a capacity for TNF-α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. In the current study, the suppressive effects of EsH on phorbol 12-myristate 13-acetate (PMA)-induced cell migration were examined in murine colon Cancer CT26 cells and human colon Cancer HCT116 cells. Interestingly, the transwell assay and wound healing show that EsH suppresses the PMA-induced migration and growth potential of HCT116 and CT26 colon Cancer cells, respectively. EsH dose-dependently suppressed matrix metalloproteinases-9 (MMP-9) expression that was upregulated upon PMA treatment in messenger RNA levels and protein secretion. Since the expression of MMP-9 is correlated with nuclear factor-κB (NF-κB) signaling, it has been examined whether EsH inhibits PMA-induced IκB phosphorylation that leads to the suppression of NK-κB nuclear translocation. EsH repressed the phosphorylation level of JNK, but not extracellular signal-regulated kinase and p38 signaling when the cells were treated with PMA. Overall, these results demonstrated that EsH could suppress Cancer migration through blockage of the JNK1/2 and NF-κB signaling-mediated MMP-9 expression.

Keywords

cancer; cancer growth; colon cancer; migration; mitogen-activated protein kinase-9.

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