2. Academic Validation
  3. SRPIN340 protects heart muscle from oxidative damage via SRPK1/2 inhibition-mediated AKT activation

SRPIN340 protects heart muscle from oxidative damage via SRPK1/2 inhibition-mediated AKT activation

  • Biochem Biophys Res Commun. 2019 Feb 26;510(1):97-103. doi: 10.1016/j.bbrc.2019.01.054.
Jian Huang 1,  Yaqun Zhou 2,  Xiaoyu Xue 2,  Liudan Jiang 1,  Jimin Du 1,  Yingyu Cui 3,  Hong Zhao 4
Affiliations

Affiliations

  • 1 Key Laboratory of Arrhythmias of the Ministry of Education of China, Tongji University School of Medicine, Shanghai 200120, China; Institute of Medical Genetics, Tongji University, Shanghai 200092, China; Department of Cardiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
  • 2 Key Laboratory of Arrhythmias of the Ministry of Education of China, Tongji University School of Medicine, Shanghai 200120, China; Institute of Medical Genetics, Tongji University, Shanghai 200092, China.
  • 3 Key Laboratory of Arrhythmias of the Ministry of Education of China, Tongji University School of Medicine, Shanghai 200120, China; Institute of Medical Genetics, Tongji University, Shanghai 200092, China; Department of Pathology and Pathophysiology, Tongji University School of Medicine, Shanghai 200092, China.
  • 4 Department of Pediatrics, Tongji Hospital, Tongji University, Shanghai 200120, China. Electronic address: zhaohong_tj@163.com.
PMID: 30661787 DOI: 10.1016/j.bbrc.2019.01.054
Abstract

SRPIN340, a selective serine-arginine protein kinase 1/2 (SRPK1/2) inhibitor, has been shown to have Antiviral and anti-angiogenesis effects. However, its role in the heart is unknown. The present study explored the role of SRPIN340 in myocardial protection and the related mechanisms. During challenge with H2O2, cardiomyocytes (CMs) pretreated with SRPIN340 showed strikingly more injury tolerance, which was manifested as reduced Lactate Dehydrogenase (LDH) release and lower apoptotic index. Further research showed that SRPIN340 activated Akt under basal conditions, and Akt inhibition abolished the protective effects of SRPIN340 treatment during H2O2 stress. The protective effect of SRPIN340 was also demonstrated in perfused rat hearts subjected to ischemia/reperfusion (I/R). Collectively, our results reveal the beneficial effects of SRPIN340 against H2O2-induced oxidative damage in CMs and I/R-induced injury in a Langendorff heart model, supporting a potential application of SRPIN340 in the clinically relevant context of reperfusion. The effectiveness of SRPIN340 may be attributed to Akt signal activation.

Keywords

AKT; Apoptosis; Cardiomyocyte; Ischemia-reperfusion; Oxidative injury; SRPIN340.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z