2. Academic Validation
  3. Effects of cooking oil fume derived fine particulate matter on blood vessel formation through the VEGF/VEGFR2/MEK1/2/ERK1/2/mTOR pathway in human umbilical vein endothelial cells

Effects of cooking oil fume derived fine particulate matter on blood vessel formation through the VEGF/VEGFR2/MEK1/2/ERK1/2/mTOR pathway in human umbilical vein endothelial cells

  • Environ Toxicol Pharmacol. 2019 Jul;69:112-119. doi: 10.1016/j.etap.2019.04.008.
Furong Zhu 1,  Han Cheng 1,  Ruoqian Lei 1,  Chaowei Shen 1,  Jie Liu 1,  Lijuan Hou 2,  Chao Zhang 1,  Yachun Xu 1,  Rui Ding 3,  Jiyu Cao 4
Affiliations

Affiliations

  • 1 Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, 81Meishan Road, Hefei, Anhui, 230032, China.
  • 2 Xuzhou Centre for Disease Control and Prevention of Jiangsu, China.
  • 3 Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, 81Meishan Road, Hefei, Anhui, 230032, China. Electronic address: kilthy@ahmu.edu.cn.
  • 4 Department of Occupational Health and Environment Health, School of Public Health, Anhui Medical University, 81Meishan Road, Hefei, Anhui, 230032, China. Electronic address: qshq@163.com.
PMID: 31026735 DOI: 10.1016/j.etap.2019.04.008
Abstract

In China, cooking oil fume derived fine particulate matter (COF-derived PM2.5) is a principal source of indoor air pollution. Here, we investigated cytotoxicity of COF-derived PM2.5, as well as the roles of VEGF, VEGFR2/KDR/Flk-1, MEK1/2, ERK1/2, and mTOR cascade in the inhibitory effects of COF-derived PM2.5, on angiogenesis in human umbilical vein endothelial cells (HUVECs). After exposure to COF-derived PM2.5, cell viability and tube formation, as well as protein and mRNA levels of VEGF, VEGFR2/KDR/Flk-1, MEK1/2, ERK1/2, and mTOR in HUVECs were measured. Cell viability and number of tubes reduced dose-dependently after COF-derived PM2.5 and SU5416 treatment. In addition, SU5416 and VEGF significantly affected tube formation. The protein and mRNA levels of VEGF, VEGFR2/KDR/Flk-1, MEK1/2, ERK1/2, and mTOR all tended to reduce with the increase of COF-derived PM2.5 concentrations. These findings demonstrate that VEGF, VEGFR2/KDR/Flk-1, MEK1/2, ERK1/2, and mTOR play key roles in COF-derived PM2.5 induced inhibition of angiogenesis in HUVECs.

Keywords

Angiopoiesis; Cooking oil fume; Human umbilical vein endothelial cells; Low birth weight; PM(2.5); Vascular endothelial growth factor.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-10374
    99.96%, Flk-1/KDR抑制剂
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