2. Academic Validation
  3. CD93 is a cell surface lectin receptor involved in the control of the inflammatory response stimulated by exogenous DNA

CD93 is a cell surface lectin receptor involved in the control of the inflammatory response stimulated by exogenous DNA

  • Immunology. 2019 Oct;158(2):85-93. doi: 10.1111/imm.13100.
Brice Nativel 1 2,  Stéphane Ramin-Mangata 1 2,  Rudy Mevizou 1,  Audrey Figuester 1,  Jessica Andries 1,  Thomas Iwema 1,  Nobunao Ikewaki 3,  Philippe Gasque 1 4 5,  Wildriss Viranaïcken 1 4
Affiliations

Affiliations

  • 1 GRI, Groupe de recherche en immunopathologie, EA4517, Université de la Réunion, Saint-Denis, France.
  • 2 Université de La Réunion, INSERM 1188, Diabète athérothombose Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France.
  • 3 Laboratories of Clinical Immunology, Department of Animal Pharmaceutical Science, Welfare School of Pharmaceutical Sciences, Kyushu University of Health, Miyazaki, Japan.
  • 4 Université de La Réunion, INSERM 1187, CNRS, 9192, IRD 249, UM 134 Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Saint-Denis de La Réunion, France.
  • 5 Laboratoire d'Immunologie Clinique et Expérimentale, ZOI (LICE-OI). CHU site Bellepierre, Saint-Denis de La Réunion, France.
PMID: 31335975 DOI: 10.1111/imm.13100
Abstract

Bacterial DNA contains CpG oligonucleotide (ODN) motifs to trigger innate immune responses through the endosomal receptor Toll-like Receptor 9 (TLR9). One of the cell surface receptors to capture and deliver microbial DNA to intracellular TLR9 is the C-type lectin molecule DEC-205 through its N-terminal C-type lectin-like domain (CTLD). CD93 is a cell surface protein and member of the lectin group XIV with a CTLD. We hypothesized that CD93 could interact with CpG motifs, and possibly serve as a novel receptor to deliver Bacterial DNA to endosomal TLR9. Using ELISA and tryptophan fluorescence binding studies we observed that the soluble histidine-tagged CD93-CTLD was specifically binding to CpG ODN and Bacterial DNA. Moreover, we found that CpG ODN could bind to CD93-expressing IMR32 neuroblastoma cells and induced more robust interleukin-6 secretion when compared with mock-transfected IMR32 control cells. Our data argue for a possible contribution of CD93 to control cell responsiveness to Bacterial DNA in a manner reminiscent of DEC-205. We postulate that CD93 may act as a receptor at plasma membrane for DNA or CpG ODN and to grant delivery to endosomal TLR9.

Keywords

C-type lectin-like domain; CD93; CpG oligonucleotide; Toll-like receptor 9; bacterial DNA; inflammation.

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