2. Academic Validation
  3. Prostaglandin E1 attenuates high glucose-induced apoptosis in proximal renal tubular cells by inhibiting the JNK/Bim pathway

Prostaglandin E1 attenuates high glucose-induced apoptosis in proximal renal tubular cells by inhibiting the JNK/Bim pathway

  • Acta Pharmacol Sin. 2020 Apr;41(4):561-571. doi: 10.1038/s41401-019-0314-9.
Yu-Han Zhang  # 1,  Ya-Qin Zhang  # 1 2,  Cong-Cong Guo 3 4,  Li-Kang Wang 1 3,  Yu-Jiao Cui 1 3,  Jian-Jun Dong 5,  Lin Liao 6 7
Affiliations

Affiliations

  • 1 Division of Endocrinology, Department of Internal Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Ji-nan, 250014, China.
  • 2 Division of Endocrinology, Department of Internal Medicine, Wuhan Third Hospital, Wuhan, 430060, China.
  • 3 First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Ji-nan, 250355, China.
  • 4 Department of Endocrinology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji-nan, 250011, China.
  • 5 Division of Endocrinology, Department of Internal Medicine, Qilu Hospital of Shandong University, Ji-nan, 250012, China. cwc_ll@sdu.edu.cn.
  • 6 Division of Endocrinology, Department of Internal Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Ji-nan, 250014, China. liaolin@sdu.edu.cn.
  • 7 Division of Endocrinology, Department of Internal Medicine, the First Affiliated Hospital of Shandong First Medical University, Ji-nan, 250014, China. liaolin@sdu.edu.cn.
  • # Contributed equally.
PMID: 31685975 DOI: 10.1038/s41401-019-0314-9
Abstract

Proximal renal tubular damage is a critical process underlying diabetic kidney disease (DKD). Our previous study shows that prostaglandin E1 (PGE1) reduces the Apoptosis of renal tubular cells in DKD rats. But its underlying mechanisms remain unclear. In this study we investigated the protective effects of PGE1 in DKD rats and high glucose (HG, 30 mM)-treated HK-2 proximal tubular cells. Four weeks after uninephrectomized streptozotocin-induced diabetic rats were established, the DKD rats were administered PGE1 (10 µg· kg-1· d-1, iv.) for 10 consecutive days. We showed that PGE1 administration did not change blood glucose levels, but alleviated diabetic kidney injury in the DKD rats, evidenced by markedly reduced proteinuria and renal tubular Apoptosis. In the in vitro experiments, PGE1 (0.1-100 µM) significantly enhanced HG-reduced HK-2 cell viability. In HG-treated HK-2 cells, PGE1 (10 µM) significantly suppressed the c-Jun N-terminal kinase (JNK) and the mitochondrial apoptosis-related protein expressions such as Bim, Bax, Caspase-3 and cleaved caspase-3; similar changes were also observed in the kidney of PGE1-treated DKD rats. By using two pharmacological tools-JNK activator anisomycin (AM) and JNK Inhibitor SP600125, we revealed that PGE1 blocked HG-triggered activation of JNK/Bim pathway in HK-2 cells; JNK was an upstream regulator of Bim. In conclusion, our results demonstrate that the nephroprotective effects of PGE1 against Apoptosis of proximal renal tubule in DKD rats via suppressing JNK-related Bim signaling pathway.

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