2. Academic Validation
  3. Anti-inflammatory and antioxidant effects of Chaetoglobosin Vb in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways

Anti-inflammatory and antioxidant effects of Chaetoglobosin Vb in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways

  • Food Chem Toxicol. 2021 Jan:147:111915. doi: 10.1016/j.fct.2020.111915.
Hongli Zhang 1,  Qingfeng Guo 2,  Zhenhua Liang 1,  Mengke Wang 1,  Baoguang Wang 1,  Dongxiao Sun-Waterhouse 3,  Geoffrey I N Waterhouse 3,  Jinmei Wang 4,  Changyang Ma 5,  Wenyi Kang 6
Affiliations

Affiliations

  • 1 National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, Henan Province, 475004, China.
  • 2 Zhengzhou City Key Laboratory of Medicinal Resources Research, Huanghe Science and Technology College, Zhengzhou, 450063, China.
  • 3 School of Chemical Sciences, University of Auckland, Auckland, 1142, New Zealand.
  • 4 National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, Henan Province, 475004, China. Electronic address: wangjinmeiscp@126.com.
  • 5 National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, Henan Province, 475004, China. Electronic address: macaya1024@sina.com.
  • 6 National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, Henan Province, 475004, China. Electronic address: kangweny@hotmail.com.
PMID: 33285210 DOI: 10.1016/j.fct.2020.111915
Abstract

There are few reports on the biological activities of chaetoglobosin Vb (Cha Vb) (a cytochalasin alkaloid). In this study, we investigated the molecular mechanisms underlying the anti-inflammatory and antioxidant effects of Cha Vb in the RAW264.7 cells stimulated lipopolysaccharide (LPS). LPS stimulation-induced oxidative stress (i.e. increase production of Reactive Oxygen Species (ROS) and decreased expression of antioxidant superoxide dismutase (SOD)) was suppressed after a Cha Vb treatment. Cha Vb could significantly inhibit the upregulated expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene and protein induced by LPS whilst attenuating the production of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. Such antioxidant and anti-inflammatory effects were achieved through the TLR4-mediated MyD88-dependent signaling pathways (via suppressing the phosphorylation of p38, ERK, JNK MAPK and translocation of the NF-κB p65 subunit into nucleus), and the TRIF-dependent signaling pathways (via reducing IFN-β release without inhibiting interferon-regulated factor 3 (IRF3) and IRF7). At 25-100 μM (a concentration range with no cytotoxicity), Cha Vb dose-dependently influenced SOD Enzyme activity and phosphorylation of p38, ERK1/2 and JNK, and at 100 μM, likely exerted the greatest inhibition towards LPS-induced oxidative stress and inflammatory response via the MAPK and NF-κB signaling pathway.

Keywords

Anti-inflammation; Antioxidant; Chaetoglobosin V(b); RAW264.7 cells; Signaling pathway.

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