2. Academic Validation
  3. Fast Stereolithography Printing of Large-Scale Biocompatible Hydrogel Models

Fast Stereolithography Printing of Large-Scale Biocompatible Hydrogel Models

  • Adv Healthc Mater. 2021 May;10(10):e2002103. doi: 10.1002/adhm.202002103.
Nanditha Anandakrishnan 1,  Hang Ye 2,  Zipeng Guo 2,  Zhaowei Chen 1,  Kyle I Mentkowski 1 3,  Jennifer K Lang 1 3 4,  Nika Rajabian 5,  Stelios T Andreadis 1 5,  Zhen Ma 6,  Joseph A Spernyak 7,  Jonathan F Lovell 1,  Depeng Wang 1,  Jun Xia 1,  Chi Zhou 2,  Ruogang Zhao 1
Affiliations

Affiliations

  • 1 Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.
  • 2 Department of Industrial and Systems Engineering, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.
  • 3 Department of Medicine, Division of Cardiology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, 14203, USA.
  • 4 VA WNY Healthcare System, Buffalo, NY, 14215, USA.
  • 5 Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.
  • 6 Department of Biomedical and Chemical Engineering, Syracuse Biomaterials Institute, Syracuse University, Syracuse, NY, 13244, USA.
  • 7 Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA.
PMID: 33586366 DOI: 10.1002/adhm.202002103
Abstract

Large size cell-laden hydrogel models hold great promise for tissue repair and organ transplantation, but their fabrication using 3D bioprinting is limited by the slow printing speed that can affect the part quality and the biological activity of the encapsulated cells. Here a fast hydrogel stereolithography printing (FLOAT) method is presented that allows the creation of a centimeter-sized, multiscale solid hydrogel model within minutes. Through precisely controlling the photopolymerization condition, low suction force-driven, high-velocity flow of the hydrogel prepolymer is established that supports the continuous replenishment of the prepolymer solution below the curing part and the nonstop part growth. The rapid printing of centimeter-sized hydrogel models using FLOAT is shown to significantly reduce the part deformation and cellular injury caused by the prolonged exposure to the environmental stresses in conventional 3D printing methods. Embedded vessel networks fabricated through multiscale printing allows media perfusion needed to maintain the high cellular viability and metabolic functions in the deep core of the large-sized models. The endothelialization of this vessel network allows the establishment of barrier functions. Together, these studies demonstrate a rapid 3D hydrogel printing method and represent a first step toward the fabrication of large-sized engineered tissue models.

Keywords

3D bioprinting; continuous printing; endothelialization; hydrogels; stereolithography.

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