1. Academic Validation
  2. ADAM17 is an essential attachment factor for classical swine fever virus

ADAM17 is an essential attachment factor for classical swine fever virus

  • PLoS Pathog. 2021 Mar 8;17(3):e1009393. doi: 10.1371/journal.ppat.1009393.
Fei Yuan 1 Dandan Li 2 Changyao Li 3 Yanan Zhang 2 Hao Song 4 Suhua Li 2 Hongkui Deng 5 George F Gao 1 Aihua Zheng 2 6 7
Affiliations

Affiliations

  • 1 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
  • 2 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • 3 College of Veterinary Medicine, China Agricultural University, Beijing, China.
  • 4 Research Network of Immunity and Health (RNIH), Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, China.
  • 5 Peking University Stem Cell Research Center, Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
  • 6 Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, China.
  • 7 College of Life Science, Henan Normal University, Xinxiang, China.
Abstract

Classical swine fever virus (CSFV) is an important pathogen in the swine industry. Virion attachment is mediated by envelope proteins Erns and E2, and E2 is indispensable. Using a pull-down assay with soluble E2 as the bait, we demonstrated that ADAM17, a disintegrin and metalloproteinase 17, is essential for CSFV entry. Loss of ADAM17 in a permissive cell line eliminated E2 binding and viral entry, but compensation with pig ADAM17 cDNA completely rescued these phenotypes. Similarly, ADAM17 silencing in primary porcine fibroblasts significantly impaired virus Infection. In addition, human and mouse ADAM17, which is highly homologous to pig ADAM17, also mediated CSFV entry. The metalloproteinase domain of ADAM17 bound directly to E2 protein in a zinc-dependent manner. A surface exposed region within this domain was mapped and shown to be critical for CSFV entry. These findings clearly demonstrate that ADAM17 serves as an essential attachment factor for CSFV.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-10293
    98.09%, ADAM10/17 抑制剂
    MMP