1. Academic Validation
  2. Tomatidine provides mitophagy-independent neuroprotection after ischemic injury

Tomatidine provides mitophagy-independent neuroprotection after ischemic injury

  • FEBS Open Bio. 2021 Sep;11(9):2647-2654. doi: 10.1002/2211-5463.13265.
Yu-Ting Wang 1 Li-Na Zhang 1 Xiao-Cui Lyu 1 Yue Li 2 Anil Ahsan 2 Zikai Feng 2 Xiangnan Zhang 2
Affiliations

Affiliations

  • 1 Department of Anesthesiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
  • 2 Institute of Pharmacology and Toxicology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Abstract

Cerebral ischemia is one of the leading causes of human mortality and disability worldwide. The treatment of cerebral ischemia is refractory due to its short therapeutic window and lack of effective clinical drugs. Mitophagy, the autophagic elimination of damaged mitochondria, attenuates neuronal injury in cerebral ischemia, indicating the potential of Mitophagy inducers as therapies for cerebral ischemia. We previously determined that, by enhancing Autophagy flux, the steroidal alkaloid tomatidine can function as a neuroprotective agent against ischemic injury. However, its effects on Mitophagy remain unknown. For this purpose, neuroblastoma cell lines Neuro-2a and SH-SY5Y were subjected to ischemic injury induced by oxygen-glucose deprivation/reperfusion (OGD/R) and then treated with tomatidine. OGD/R induced a general decrease of cellular contents, and this study revealed that tomatidine had no impact on Mitophagy. In addition, tomatidine did not affect mitochondrial contents, including translocase of outer mitochondrial membrane 20 and voltage-dependent anion channel 1, in either OGD/R-treated or intact SH-SY5H cells. Our results indicate that tomatidine exhibits its neuroprotective effects by enhancing Autophagy, but in a potentially mitophagy-independent manner, and provide insights for further investigation into its mechanism(s) and potential therapeutic use against cerebral ischemia.

Keywords

autophagy; cerebral ischemia; mitophagy; tomatidine.

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