1. Academic Validation
  2. Evaluation of the immunomodulatory effects of C9-13-CPs in macrophages

Evaluation of the immunomodulatory effects of C9-13-CPs in macrophages

  • Acta Biochim Biophys Sin (Shanghai). 2021 Aug 31;53(9):1154-1165. doi: 10.1093/abbs/gmab094.
Zimeng Xue 1 Jianbo Zhu 1 Xia Wang 1 Chunlei Yang 1 Zhengwei Fu 1
Affiliations

Affiliation

  • 1 College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
Abstract

Short-chain chlorinated paraffins (SCCPs) have been listed as a new class of persistent organic pollutants by the Stockholm Convention. SCCPs exhibit carcinogenic-, endocrine-, and metabolism-disrupting effects. However, the knowledge of the immunomodulatory effects of SCCPs and their underlying mechanisms, especially in specific immune cells, remains limited. In addition to SCCPs, C9-13-CPs have also been detected in humans. In this study, murine RAW264.7 macrophages were exposed to C9-13-CPs at environmentally relevant concentrations to investigate whether or how C9-13-CPs exhibit immunomodulatory effects. The results showed that the exposure of RAW264.7 cells to C9-13-CPs increased cell viability, as assayed by MTT analysis at 490 nm, and also promoted cell proliferation, as indicated by EdU uptake assay, which was measured at excitation and emission wavelengths of 488 and 512 nm, respectively. In addition, exposure to C9-13-CPs not only led to elevated ATP level and intracellular Ca2+ level but also caused AMPK signaling activation and NF-κB signaling inhibition. Moreover, molecular docking showed that the β2-AR receptor could bind to C9-13-CPs. Taken together, these results suggest that the immune dysfunction of RAW264.7 cells caused by C9-13-CPs is closely related to the β2-AR/AMPK/NF-κB signaling axis.

Keywords

SCCPs, C9-13-CPs, RAW264.7 cells; immunomodulatory cytokines; immunotoxicity.

Figures
Products
我们的 Cookie 政策

我们使用 Cookies 和类似技术以提高网站的性能和提升您的浏览体验,部分功能也使用 Cookies 帮助我们更好地理解您的需求,为您提供相关的服务。 如果您有任何关于我们如何处理您个人信息的疑问,请阅读我们的《隐私声明》