2. Academic Validation
  3. Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton

Nanoplastics aggravate the toxicity of arsenic to AGS cells by disrupting ABC transporter and cytoskeleton

  • Ecotoxicol Environ Saf. 2021 Dec 20;227:112885. doi: 10.1016/j.ecoenv.2021.112885.
Peichun Lin 1,  Yitao Guo 2,  Lei He 2,  Xiuchun Liao 2,  Xueru Chen 2,  Liuying He 2,  Zifan Lu 3,  Zhong-Ji Qian 1,  Chunxia Zhou 4,  Pengzhi Hong 4,  Shengli Sun 2,  Chengyong Li 5
Affiliations

Affiliations

  • 1 School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China.
  • 2 School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China.
  • 3 School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China. Electronic address: quinnlzf@163.com.
  • 4 Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China; School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, PR China.
  • 5 School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang 524088, PR China; Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, PR China. Electronic address: cyli@gdou.edu.cn.
PMID: 34634601 DOI: 10.1016/j.ecoenv.2021.112885
Abstract

The coexistence of nanoplastics (NPs) and pollutants such as arsenic (As) has become an unignorable environmental problem. However, there is still a considerable knowledge gap about the impact of NPs and pollutants on human health risks. In this study, the human gastric adenocarcinoma (AGS) cells were used as a model to investigate the toxicity of NPs with different particle sizes and As by MTT assay, western blotting, immunofluorescence and so on. The results showed that 20 nm (8 μg/mL), 50 nm (128 μg/mL), 200 nm (128 μg/mL), 500 nm (128 μg/mL), 1000 nm (128 μg/mL) polystyrene (PS) did not affect cell viability, ROS, intracellular calcium and activate Apoptosis pathway in AGS cells. However, noncytotoxic concentration of NPs enhanced the cytotoxicity and intracellular accumulation of As. NPs destroys the fluidity of cell membrane and Cytoskeleton, inhibits the activity of ABC transporter, and leads to the accumulation of As in cells. This work highlights that the damage caused by NPs, especially at the level of noncytotoxicity, joint with As cannot be ignored and provides a specific toxicological mechanism of NPs accompanied by exposure to As.

Keywords

ABC transporter; AGS cells; Arsenic; Cytoskeleton; Nanoplastics.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z