2. Academic Validation
  3. Secnidazole Is a Promising Imidazole Mitigator of Serratia marcescens Virulence

Secnidazole Is a Promising Imidazole Mitigator of Serratia marcescens Virulence

  • Microorganisms. 2021 Nov 11;9(11):2333. doi: 10.3390/microorganisms9112333.
Ahdab N Khayyat 1,  Hisham A Abbas 2,  Maan T Khayat 1,  Moataz A Shaldam 3,  Momen Askoura 2,  Hani Z Asfour 4,  El-Sayed Khafagy 5 6,  Amr S Abu Lila 7 8,  Ahmed N Allam 9,  Wael A H Hegazy 2
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • 2 Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
  • 3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafr El-Sheikh 33511, Egypt.
  • 4 Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • 5 Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
  • 6 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 41552, Egypt.
  • 7 Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail 81442, Saudi Arabia.
  • 8 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
  • 9 Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt.
PMID: 34835458 DOI: 10.3390/microorganisms9112333
Abstract

Serratia marcescens is an opportunistic pathogen that causes diverse nosocomial infections. S. marcescens has developed considerable resistance to different Antibiotics and is equipped with an armory of virulence factors. These virulence factors are regulated in S. marcescens by an intercellular communication system termed quorum sensing (QS). Targeting Bacterial virulence and QS is an interesting approach to mitigating Bacterial pathogenesis and overcoming the development of resistance to antimicrobials. In this study, we aimed to evaluate the anti-virulence activities of secnidazole on a clinical isolate of S. marcescens. The effects of secnidazole at sub-inhibitory concentrations (sub-MICs) on virulence factors, swarming motility, biofilm formation, proteases, hemolysin activity, and prodigiosin production were evaluated in vitro. Secnidazole's protective activity against S. marcescens pathogenesis was assessed in vivo in mice. Furthermore, a molecular docking study was conducted to evaluate the binding ability of secnidazole to the S. marcescens SmaR QS receptor. Our findings showed that secnidazole at sub-MICs significantly reduced S. marcescens virulence factor production in vitro and diminished its pathogenesis in mice. The insilico docking study revealed a great ability of secnidazole to competitively hinder the binding of the autoinducer to the SmaR QS receptor. In conclusion, secnidazole is a promising anti-virulence agent that may be used to control infections caused by S. marcescens.

Keywords

Serratia marcescens; anti-quorum sensing agents; anti-virulence agents; pathogenesis; quorum sensing; secnidazole; virulence factors.

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