2. Academic Validation
  3. Microwell bag culture for large-scale production of homogeneous islet-like clusters

Microwell bag culture for large-scale production of homogeneous islet-like clusters

  • Sci Rep. 2022 Mar 25;12(1):5221. doi: 10.1038/s41598-022-09124-w.
Ryo Suenaga  # 1,  Shuhei Konagaya  # 2,  Junji Yamaura 3 4,  Ryo Ito 2,  Satoshi Tanaka 5,  Yoichi Ishizaki 5,  Taro Toyoda 6 7
Affiliations

Affiliations

  • 1 Corporate Research & Development, Toyo Seikan Group Holdings, Ltd., Yokohama, Japan. ryou_suenaga@tskg-hd.com.
  • 2 iPSC-derived Pancreatic Islet Cells (iPIC) Therapy Department, Orizuru Therapeutics, Inc., Fujisawa, Kanagawa, Japan.
  • 3 Cell Therapies, Pharmaceutical Science, Takeda Pharmaceutical Company Limited, Fujisawa, Kanagawa, Japan.
  • 4 Takeda-CiRA Joint Program for iPS Cell Applications (T-CiRA), Fujisawa, Kanagawa, Japan.
  • 5 Corporate Research & Development, Toyo Seikan Group Holdings, Ltd., Yokohama, Japan.
  • 6 Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. t.toyoda@cira.kyoto-u.ac.jp.
  • 7 Takeda-CiRA Joint Program for iPS Cell Applications (T-CiRA), Fujisawa, Kanagawa, Japan. t.toyoda@cira.kyoto-u.ac.jp.
  • # Contributed equally.
PMID: 35338209 DOI: 10.1038/s41598-022-09124-w
Abstract

Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 105-106 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to be overcome for its clinical use. In this study, we aimed to develop a novel bag culture method for the production of uniform cell clusters on a large scale (105-106 clusters). We prepared small-scale culture bags (< 105 clusters) with microwells at the bottom and optimized the conditions for producing uniform-sized clusters in the bag using undifferentiated induced pluripotent stem cells (iPSCs). Subsequently, we verified the suitability of the bag culture method using iPSC-derived pancreatic islet cells (iPICs) and successfully demonstrate the production of 6.5 × 105 uniform iPIC clusters using a large-scale bag. In addition, we simplified the pre- and post-process of the culture-a degassing process before cell seeding and a cluster harvesting process. In conclusion, compared with conventional methods, the cluster production method using bags exhibits improved scalability, sterility, and operability for both clinical and research use.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z