2. Academic Validation
  3. Analysis of the Antioxidant Composition of Low Molecular Weight Metabolites from the Agarolytic Bacterium Alteromonas macleodii QZ9-9: Possibilities for High-Added Value Utilization of Macroalgae

Analysis of the Antioxidant Composition of Low Molecular Weight Metabolites from the Agarolytic Bacterium Alteromonas macleodii QZ9-9: Possibilities for High-Added Value Utilization of Macroalgae

  • Antioxidants (Basel). 2022 Oct 3;11(10):1977. doi: 10.3390/antiox11101977.
Xinyi Wang 1 2 3 4,  Ziqiao Feng 1 2 3 4,  Chenhui Li 1 2 3 4,  Xiaoni Cai 1 2 3 4,  Hao Long 1 2 3 4,  Xiang Zhang 1 2 3 4,  Aiyou Huang 1 2 3 4,  Yanhua Zeng 1 2 3 4,  Wei Ren 1 2 3 4,  Zhenyu Xie 1 2 3 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, China.
  • 2 Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, Hainan University, Haikou 570228, China.
  • 3 College of Marine Sciences, Hainan University, Haikou 570228, China.
  • 4 Laboratory of Development and Utilization of Marine Microbial Resource, Hainan University, Haikou 570228, China.
PMID: 36290700 DOI: 10.3390/antiox11101977
Abstract

Agar accounts for ~60% of the dry weight of some red macroalgae, and the breakdown of this kind of polysaccharide releases high-value compounds; therefore, the resource utilization of agar is of great significance to improve the added value of these macroalgae. Herein, Alteromonas macleodii QZ9-9 isolated from tropical Gracilaria hainanensis in Hainan Island was characterized as an agarolytic bacterium, which displayed a high agar-degrading activity. The highest diameters of the degradation zones of the A. macleodii QZ9-9 and its extracellular-agarase (12.16 U/mL) were 41.46 mm and 22.89 mm, respectively, and the first-order degradation rate constants of those were 0.02 h-1 and 0.77 U-1, respectively. Importantly, the fermentation products of A. macleodii QZ9-9 exhibited antioxidant activity, and the peak of DPPH scavenging activity of 50 h fermentation products of this strain was up to 50.79% in the reaction for 1 h; the DPPH scavenging activity of low molecule metabolites (≤3 kDa) in particular was up to ~85.85%. A total of 766 metabolites were detected in the low molecule metabolites by metabolomics. The peptide-like metabolites, such as prolyl-histidine, isoleucyl-histidine, isoleucyl-proline and arginyl-proline, and the antioxidant maculosin were found in the top 20 metabolites with relatively high abundance. Additionally, the antioxidant activity of maculosin was further verified in this work. We concluded that the low molecule metabolites of A. macleodii QZ9-9 with relatively high antioxidant activity are interesting candidates for preparing desirable non-toxic Antioxidants, thereby facilitating the high value-added utilization of macroalgae in the fields of cosmetic, food preservation, and pharmaceutical industries.

Keywords

agarase; agarolytic bacterium; antioxidant activity; antioxidant peptides; macroalgae; maculosin.

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