2. Academic Validation
  3. Isoprenylation modification is required for HIPP1-mediated powdery mildew resistance in wheat

Isoprenylation modification is required for HIPP1-mediated powdery mildew resistance in wheat

  • Plant Cell Environ. 2022 Nov 1. doi: 10.1111/pce.14479.
Zongkuan Wang 1 2,  Heng Zhang 1 2 3,  Yingbo Li 1 2 4,  Yiming Chen 1,  Xiong Tang 1,  Jia Zhao 1,  Feifei Yu 1,  Haiyan Wang 1,  Jin Xiao 1,  Jia Liu 1,  Xu Zhang 1,  Li Sun 1,  Qi Xie 1,  Xiue Wang 1
Affiliations

Affiliations

  • 1 State Key Lab of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, College of Agriculture, Nanjing Agricultural University/JCIC-MCP, Nanjing, Jiangsu, China.
  • 2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
  • 3 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
  • 4 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
PMID: 36319595 DOI: 10.1111/pce.14479
Abstract

Powdery mildew (Pm), caused by Blumeria graminis f.sp. tritici (Bgt), is one of the most important wheat diseases. Heavy-metal-associated isoprenylated plant protein (HIPP1) has been proved playing important roles in response to biotic and a biotic stress. In present study, we proved HIPP1-V from Haynalidia villosa is a positive regulator in Pm resistance. HIPP1-V was rapidly induced by Bgt. Transiently or stably heterologous overexpressing HIPP1-V in wheat suppressed the haustorium formation and enhanced Pm resistance. HIPP1-V isoprenylation was critical for plasma membrane (PM) localization, interaction with E3-ligase CMPG1-V and function in Pm resistance. Bgt Infection recruited the isoprenylated HIPP1-V and CMPG1s on PM; blocking the HIPP1 isoprenylation reduced such recruitment and compromised the resistance of OE-CMPG1-V and OE-HIPP1-V. Overexpressing HIPP1-VC148G could not enhance Pm resistance. These indicated the Pm resistance was dependent on HIPP1-V's isoprenylation. DGEs related to the ROS and SA pathways were remarkably enriched in OE-HIPP1-V, revealing their involvement in Pm resistance. Our results provide evidence on the important role of protein isoprenylation in plant defense.

Keywords

Triticum aestivum L.; heavy-metal-associated protein; plant defence signalling; protein isoprenylation modification.

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