2. Academic Validation
  3. OECs Prevented Neuronal Cells from Apoptosis Partially Through Exosome-derived BDNF

OECs Prevented Neuronal Cells from Apoptosis Partially Through Exosome-derived BDNF

  • J Mol Neurosci. 2022 Dec 17. doi: 10.1007/s12031-022-02097-5.
Zhe Chen # 1 2,  Hong Fan # 2 3,  Zi-Yi Chen # 4,  Chao Jiang 2,  Ming-Zhe Feng 2,  Xin-Yu Guo 2,  Hao Yang 5,  Ding-Jun Hao 6 7
Affiliations

Affiliations

  • 1 Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
  • 2 Shaanxi Spine Medicine Research Center, Department of Spine Surgery, Translational Medicine Center, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710054, China.
  • 3 Department of Neurology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.
  • 4 Department of Endocrinology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.
  • 5 Shaanxi Spine Medicine Research Center, Department of Spine Surgery, Translational Medicine Center, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710054, China. yanghao.71_99@yahoo.com.
  • 6 Department of Orthopedics, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China. haodingjun@126.com.
  • 7 Shaanxi Spine Medicine Research Center, Department of Spine Surgery, Translational Medicine Center, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710054, China. haodingjun@126.com.
  • # Contributed equally.
PMID: 36527597 DOI: 10.1007/s12031-022-02097-5
Abstract

It is known that Neurotrophic Factors are a major source of the neuroprotective effects of olfactory ensheathing cells (OECs). However, the form of Neurotrophic Factors that originate from OECs is not fully understood. Our previous study demonstrated that OECs could secrete exosome (OECs-Exo), which provided neuroprotection by switching the phenotype of macrophages/microglia. Considering that exosomes could also be taken up by neurons, we explored the direct effect of OECs-Exo on neuronal survival and the underlying mechanism. Electron microscopy, nano-traffic analysis, and Western blotting were applied to identify the OECs-Exo. The effect of OECs-Exo on neuronal survival was tested by flow cytometry and TUNEL staining. Western blotting and ELISA were used to detect Neurotrophic Factors in purified OECs-Exo. We first isolated OECs-Exo and found that OECs-Exo exerted protective effects on neuronal survival in response to TNF-α challenge. Brain-derived neurotrophic factor (BDNF) was then identified in OECs-Exo, and its receptor TrkB in neurons was activated by OECs-Exo treatment. Furthermore, we demonstrated that OECs prevented TNF-α-induced Apoptosis in neurons partially through exosome-derived BDNF. Our data showed that OECs attenuated TNF-α-induced Apoptosis in neurons partially through OEC-Exo-derived BDNF, which might provide a novel strategy for the neuroprotective effect of OEC-Exo-based treatment.

Keywords

BDNF; Exosomes; Neuronal survival; OECs.

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