2. Academic Validation
  3. Lateralized response of skull bone marrow via osteopontin signaling in mice after ischemia reperfusion

Lateralized response of skull bone marrow via osteopontin signaling in mice after ischemia reperfusion

  • J Neuroinflammation. 2023 Dec 9;20(1):294. doi: 10.1186/s12974-023-02980-x.
Chaoran Xu # 1,  Qia Zhang # 1,  Yi Zhang # 2,  Huaijun Chen # 2,  Tianchi Tang 2,  Junjie Wang 1,  Siqi Xia 2,  Gao Chen 3 4,  Jianmin Zhang 5 6 7 8
Affiliations

Affiliations

  • 1 Department of Neurosurgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu, Zhejiang, China.
  • 2 Department of Neurosurgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
  • 3 Department of Neurosurgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. d-chengao@zju.edu.cn.
  • 4 Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases, Zhejiang University, Hangzhou, Zhejiang, China. d-chengao@zju.edu.cn.
  • 5 Department of Neurosurgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. zjm135@zju.edu.cn.
  • 6 Department of Neurosurgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu, Zhejiang, China. zjm135@zju.edu.cn.
  • 7 Brain Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. zjm135@zju.edu.cn.
  • 8 Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases, Zhejiang University, Hangzhou, Zhejiang, China. zjm135@zju.edu.cn.
  • # Contributed equally.
PMID: 38071333 DOI: 10.1186/s12974-023-02980-x
Abstract

Skull bone marrow is thought to be an immune tissue closely associated with the central nervous system (CNS). Recent studies have focused on the role of skull bone marrow in central nervous system disorders. In this study, we performed single-cell RNA Sequencing on ipsilateral and contralateral skull bone marrow cells after experimental stroke and then performed flow cytometry and analysis of cytokine expression. Skull marrow showed lateralization in response to stroke. Lateralization is demonstrated primarily by the proliferation and differentiation of myeloid and lymphoid lineage cells in the skull bone marrow adjacent to the ischemic region, with an increased proportion of neutrophils compared to monocytes. Analysis of chemokines in the skull revealed marked differences in chemotactic signals between the ipsilateral and contralateral skull, whereas sympathetic signals innervating the skull did not affect cranial bone marrow lateralization. Osteopontin (OPN) is involved in region-specific activation of the skull marrow that promotes inflammation in the meninges, and inhibition of OPN expression improves neurological function.

Keywords

Cytokine; Lateralization; Skull bone marrow; Stroke; Sympathetic nervous system.

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