2. Academic Validation
  3. Structure-Based Design and Evaluation of Reversible KRAS G13D Inhibitors

Structure-Based Design and Evaluation of Reversible KRAS G13D Inhibitors

  • ACS Med Chem Lett. 2023 Dec 6;15(1):21-28. doi: 10.1021/acsmedchemlett.3c00478.
Christian Nilewski 1,  Sharada Labadie 1,  Binqing Wei 1,  Sushant Malhotra 1,  Steven Do 1,  Lewis Gazzard 1,  Li Liu 2,  Cheng Shao 2,  Jeremy Murray 1,  Yevgeniy Izrayelit 1,  Amy Gustafson 1,  Nicholas F Endres 1,  Fang Ma 1,  Xin Ye 1,  Jun Zou 1,  Marie Evangelista 1
Affiliations

Affiliations

  • 1 Genentech Inc., 1 DNA Way, South San Francisco, California 94080, United States.
  • 2 Pharmaron-Beijing Co. Ltd., 6 Taihe Road, BDA, Beijing 100176, P. R. China.
PMID: 38229748 DOI: 10.1021/acsmedchemlett.3c00478
Abstract

Oncogenic KRAS mutations were identified decades ago, yet the selective inhibition of specific KRAS mutant proteins represents an ongoing challenge. Recent progress has been made in targeting certain P-loop mutant proteins, in particular KRAS G12C, for which the covalent inhibition of the GDP state via the Switch II pocket is now a clinically validated strategy. Inhibition of Other KRAS mutant proteins such as KRAS G13D, on the Other hand, still requires clinical validation. The remoteness of the D13 residue relative to the Switch II pocket in combination with the solvent exposure and conformational flexibility of the D13 side chain, as well as the difficulties of targeting carboxylate residues covalently, renders this specific protein particularly challenging to target selectively. In this report, we describe the design and evaluation of potent and KRAS G13D-selective reversible inhibitors. Subnanomolar binding to the GDP state Switch II pocket and biochemical selectivity over WT KRAS are achieved by leveraging a salt bridge with D13.

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