2. Academic Validation
  3. Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress

Berberine inhibits SGIV replication by suppressing inflammatory response and oxidative stress

  • Fish Shellfish Immunol. 2024 Mar 26:109522. doi: 10.1016/j.fsi.2024.109522.
Yunxiang Jiang 1,  Chengzong Han 1,  Hannan Gong 1,  Jiatao Chen 1,  Biao Tang 1,  Min Yang 2,  Qiwei Qin 3,  Shina Wei 4
Affiliations

Affiliations

  • 1 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
  • 2 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou, 511457, China.
  • 3 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou, 511457, China. Electronic address: qinqw@scau.edu.cn.
  • 4 College of Marine Sciences, South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South China Agricultural University Fishery Research Institute, Guangzhou, 511457, China. Electronic address: weisn@scau.edu.cn.
PMID: 38548190 DOI: 10.1016/j.fsi.2024.109522
Abstract

Singapore grouper iridovirus (SGIV) is one of the major infectious diseases responsible for high mortality and huge economic losses in the grouper aquaculture industry. Berberine (BBR), a naturally occurring plant alkaloid, is a phytochemical having a variety of biological properties, such as Antiviral, antioxidant, and anti-inflammatory effects. In this work, we used an in vitro model based on Western blot, ROS fluorescence probe, and real-time quantitative PCR (qRT-PCR) to examine the Antiviral qualities of BBR against SGIV. The outcomes demonstrated that varying BBR concentrations could significantly inhibit the replication of SGIV. In addition, BBR greatly inhibited the production of genes associated with pro-inflammatory cytokines in SGIV-infected or SGIV-uninfected GS cells based on qRT-PCR data. Subsequent investigations demonstrated that BBR suppressed the expression of the promoter activity of NF-κB and NF-κB-p65 protein. Additionally, BBR reduced the phosphorylation of ERK 1/2, JNK, and p38. Furthermore, BBR also inhibits SGIV-induced ROS production by upregulating the expression of antioxidant-related genes. In conclusion, BBR is a viable therapy option for SGIV Infection due to its Antiviral properties.

Keywords

Berberine; Inflammation; Oxidative stress; SGIV.

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