2. Academic Validation
  3. Functional peptide conjugated ordered gold nanoparticles based rational electrochemical immunosensor for highly stable and selective detection of zearalenone

Functional peptide conjugated ordered gold nanoparticles based rational electrochemical immunosensor for highly stable and selective detection of zearalenone

  • Food Chem. 2024 Sep 17;463(Pt 3):141297. doi: 10.1016/j.foodchem.2024.141297.
Qingyue Li 1,  Xiya Zhang 1,  Mengtian Chen 1,  Changwei Luo 1,  Ran Yang 2,  Tong Bu 1,  Meng Dang 1,  Xianqing Huang 1,  Lianjun Song 1,  Pingan Zhang 1,  Liang Liu 3,  Yexuan Mao 4
Affiliations

Affiliations

  • 1 Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, Nongye Road, Zhengzhou, Henan 450002, PR China.
  • 2 College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, China.
  • 3 Raw Material Purchasing Center, China Tobacco Henan Industrial Limited Corporation, Yulin Road, Zhengzhou, Henan 450000, PR China.
  • 4 Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, Nongye Road, Zhengzhou, Henan 450002, PR China. Electronic address: maoyexuan@henau.edu.cn.
PMID: 39305646 DOI: 10.1016/j.foodchem.2024.141297
Abstract

To integrate antifouling properties and good sensitivity on the sensing interface can improve the applicability of an electrochemical immunosensor. These functional regions can be integrated into a single functional peptide (functPP). The rational designed three domains in functPP were the anchoring, antifouling and gold nanoparticles (AuNPs) recognizing domains. Meanwhile, the ordered AuNPs inspired by C15H23CO-RRRRR can be recognized by AuNPs recognizing domains in functPP to enhance the intensity of detecting current. In the sensing system, the anchoring domain in functPP can be immobilized on the Au electrode by AuS interaction, while the antifouling domain undergoes strong hydration with water molecules to resist matrices, and the recognizing domains can directionally capture O-AuNPs to form a functPP-O-AuNPs complex as the core sensing element. Consequently, the complex bound to the monoclonal Antibodies against zearalenone by electrostatic adsorption to develop a highly antifouling and sensitive biosensor with the ability to identify zearalenone in cereals.

Keywords

Antifouling; Electrochemical immunosensor; Ordered gold nanoparticles; Zearalenone.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z