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  3. Parallel single-cell metabolic analysis and extracellular vesicle profiling reveal vulnerabilities with prognostic significance in acute myeloid leukemia

Parallel single-cell metabolic analysis and extracellular vesicle profiling reveal vulnerabilities with prognostic significance in acute myeloid leukemia

  • Nat Commun. 2024 Dec 30;15(1):10878. doi: 10.1038/s41467-024-55231-9.
Dorian Forte 1,  Roberto Maria Pellegrino 2,  Paolo Falvo 3 4,  Paulina Garcia-Gonzalez 5,  Husam B R Alabed 2,  Filippo Maltoni 1 6,  Davide Lombardi 3 4,  Samantha Bruno 1,  Martina Barone 6,  Federico Pasini 1,  Francesco Fabbri 7,  Ivan Vannini 7,  Benedetta Donati 8,  Gianluca Cristiano 1,  Chiara Sartor 1 6,  Simona Ronzoni 9,  Alessia Ciarrocchi 8,  Sandra Buratta 2,  Lorena Urbanelli 2,  Carla Emiliani 2 10,  Simona Soverini 1,  Lucia Catani 1 6,  Francesco Bertolini 3 4,  Rafael José Argüello 5,  Michele Cavo 1 6,  Antonio Curti 11
Affiliations

Affiliations

  • 1 Department of Medical and Surgical Sciences, Institute of Hematology "L. and A. Seràgnoli", University of Bologna, Bologna, Italy.
  • 2 Department of Chemistry, Biology and Biotechnology, Biochemical and Biotechnological Sciences Section, University of Perugia, Perugia, Italy.
  • 3 Laboratory of Hematology-Oncology, European Institute of Oncology IRCCS, Milan, Italy.
  • 4 Onco-Tech Lab, European Institute of Oncology IRCCS and Politecnico di Milano, Milan, Italy.
  • 5 Aix Marseille Univ, CNRS, INSERM, CIML, Centre d'Immunologie de Marseille-Luminy, Marseille, France.
  • 6 IRCCS Azienda Ospedaliero-Universitaria di Bologna, Istituto di Ematologia "Seràgnoli", Bologna, Italy.
  • 7 Biosciences Laboratory, IRCCS Istituto Romagnolo per lo Studio dei Tumori (IRST) "Dino Amadori", Meldola, Italy.
  • 8 Laboratory of Translational Research, Azienda USL-IRCCS di Reggio Emilia, Reggio Emilia, Italy.
  • 9 Department of Experimental Oncology, European Institute of Oncology IRCCS, Milano, Italy.
  • 10 Centro di Eccellenza sui Materiali Innovativi Nanostrutturati (CEMIN), University of Perugia, Perugia, Italy.
  • 11 IRCCS Azienda Ospedaliero-Universitaria di Bologna, Istituto di Ematologia "Seràgnoli", Bologna, Italy. antonio.curti2@unibo.it.
PMID: 39738118 DOI: 10.1038/s41467-024-55231-9
Abstract

Acute myeloid leukemia (AML) is an aggressive disease with a high relapse rate. In this study, we map the metabolic profile of CD34+(CD38low/-) AML cells and the extracellular vesicle signatures in circulation from AML patients at diagnosis. CD34+ AML cells display high antioxidant glutathione levels and enhanced mitochondrial functionality, both associated with poor clinical outcomes. Although CD34+ AML cells are highly dependent on glucose oxidation and glycolysis for energy, those from intermediate- and adverse-risk patients reveal increased mitochondrial dependence. Extracellular vesicles from AML are mainly enriched in stem cell markers and express antioxidant GPX3, with their profiles showing potential prognostic value. Extracellular vesicles enhance mitochondrial functionality and dependence on CD34+ AML cells via the glutathione/GPX4 axis. Notably, extracellular vesicles from adverse-risk patients enhance leukemia cell engraftment in vivo. Here, we show a potential noninvasive approach based on liquid 'cell-extracellular vesicle' biopsy toward a redefined metabolic stratification in AML.

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