2. Academic Validation
  3. Preparation and specificity of antibodies directed toward the ribose methylated nucleotide, 2'-O-methylguanosine 5'-monophosphate

Preparation and specificity of antibodies directed toward the ribose methylated nucleotide, 2'-O-methylguanosine 5'-monophosphate

  • Biochim Biophys Acta. 1981 Sep 28;655(2):265-7. doi: 10.1016/0005-2787(81)90017-4.
B S Vold
PMID: 6269628 DOI: 10.1016/0005-2787(81)90017-4
Abstract

Antibodies have been produced that recognize the ribosome methylated nucleotide, 2'-O-methylguanosine 5'-monophosphate, pGm. Specificity was tested in a radioimmunoassay. Other ribose methylated nucleotides, deoxyguanosine, and guanosine 5'-monophosphate exhibited negligible cross-reactivity. Elements of antibody recognition, in descending order of importance, were the parent base, the methylated ribose moiety, and the phosphate group.

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