Monoamine oxidase, plasma  (Synonyms: 单胺氧化酶)

Monoamine oxidase (EC 1.4.3.4) is an enzyme composed of different polypeptides. Monoamine oxidation catalyzes the oxidative deamination of various biological amines in brain and peripheral tissues by producing hydrogen peroxide. Monoamine oxidase plays an important role in maintaining the regulation of synaptic transmission, emotional behavior and other brain functions^[1][2].

Monoamine oxidase 有一些同工酶，其中最典型的是单胺氧化酶 (MAO) A 和 MAOB 。MAOA 通常被低浓度氯吉林 (Clorgyline，HY-14197A) 抑制，MAOB 通常被低浓度的 l-盐酸司立吉林抑制^[3]。
单胺氧化酶测定
1. 溶液配制
1）底物：1.0% v/v (0.10 M) 苄胺于 67 mM 磷酸钾缓冲液中，pH 7.2。
2）酶溶液：在 67 mM 磷酸钾缓冲液 (pH 7.2) 中溶解至浓度为 10 mg/mL，2-8℃ 最多储存 1 周。
2. 测定步骤
1) 将分光光度计调节至 250 nm 和 25°C。
2) 向每个比色皿中移取 2.8 mL 67 mM Potassium phosphate buffer pH 7.2 和 0.1 mL 底物溶液。
3) 在分光光度计中孵育 3-4 分钟以达到温度平衡。
4) 添加 0.1 mL 酶溶液并记录 6-8 分钟内 A250 的增加量。根据曲线的线性部分计算 ΔA250/分钟。可能会出现 2-3 分钟的滞后。
5) 计算
Units/g = (ΔA250/min x 1000)/(13.0 x mg enzyme/mL reaction mixture)

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9001-66-5

固体

White to off-white

单胺氧化酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Shih JC, et al. Monoamine oxidase: from genes to behavior. Annu Rev Neurosci. 1999;22:197-217.  [Content Brief]

  [2]. Bortolato M, et al. Monoamine oxidase inactivation: from pathophysiology to therapeutics. Adv Drug Deliv Rev. 2008 Oct-Nov;60(13-14):1527-33.  [Content Brief]

  [3]. Youdim MB, et al. The therapeutic potential of monoamine oxidase inhibitors. Nat Rev Neurosci. 2006 Apr;7(4):295-309.  [Content Brief]