Nuclease P1  (Synonyms: 核酸酶P1)

Nuclease P1 is a single-stranded specific endonuclease, it hydrolyzes nucleic acids into 5'-mononucleotides and cleaves the single-stranded region of a double-stranded nucleic acid. Nuclease P1 is one of the most well-known single stranded specific nucleases in the field of molecular biology, it is widely used in the pharmaceutical and food industries^[1]. Nuclease P1 can be obtained by fermentation of Penicillium citrinum: through extraction process, ultrafiltration concentration, drying and purification, etc.

反应条件
pH 范围：有效pH 范围：4.0-8.0， 最适pH 范围：4.5-5.5
温度范围：有效温度范围：35°C-85°C，最适温度范围：65°C-75°C

使用方法
酵母核酸（RNA）为底物，底物浓度为2%-5%，推荐加酶量为2%-4%（按底物干物计），在最适条件下酶解时间为7 h-9 h；
酵母为底物，酵母浓度为10%-12%，核酸酶用量为0.3%-0.5%（按酵母干物计），在最适条件下酶解时间为7 h- 9 h。
注：由于应用领域和实验参数的差异，本品的实际添加方式和添加量应通过试验来确定。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

54576-84-0

固体

White to light yellow

核酸酶P1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Mitiushkina NV, et al. Preparation of Duplex Sequencing Libraries for Archival Paraffin-Embedded Tissue Samples Using Single-Strand-Specific Nuclease P1. Int J Mol Sci. 2022 Apr 21;23(9):4586.  [Content Brief]