2. Cell Cycle/DNA Damage Epigenetics Apoptosis
  3. PARP Apoptosis
  4. PARP1-IN-12

PARP1-IN-12 是一种有效的 PARP1 抑制剂，其 IC50 值为 2.99 nM。PARP1-IN-12 具有较好的抗癌细胞增殖活性，可诱导细胞凋亡并使细胞周期停滞在 G2/M 期。PARP1-IN-12 能在 BRCA 缺陷的细胞中诱导 DNA 双链断裂 (DSBs)。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

PARP1-IN-12 Chemical Structure

PARP1-IN-12 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

PARP1-IN-12 相关抗体

Top Publications Citing Use of Products

查看 PARP 亚型特异性产品:

查看所有亚型
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

PARP1-IN-12 is a potent PARP1 inhibitor with an IC50 of 2.99 nM. PARP1-IN-12 exhibits antiproliferative activity, can induce cell apoptosis and cause cycle arrest at G2/M phase. PARP1-IN-12 also can induce DNA double strand breaks (DSBs) in BRCA-deficient cells[1].

IC50 & Target

PARP-1

2.99 nM (IC50)

体外研究
(In Vitro)

PARP1-IN-12 (compound 20e) (0.1, 0.3, 1 µM; 48 h) exhibits activities of antiproliferation and selectively killing BRCA-deficient cells, can also induce DNA double strand breaks (DSBs) in BRCA-deficient cells in a concentration-dependent manner[1].
PARP1-IN-12 (10 µM, 48 h) enhances the protein levels of phosphorylated Chk1[1].
PARP1-IN-12 (1, 3, 10 µM; 48 h) activats cell cycle checkpoints, then induces G2/M arrest in BRCA-deficient cells and (1, 5, 10 µM; 96 h) also induces MDA-MB-436 cells apoptosis in a concentration-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

PARP1-IN-12 相关抗体:

Cell Proliferation Assay[1]

Cell Line: UWB1.289 (BRCA1-deficient), UWB1.289+BRCA1 (BRCA1 restored), MDA-MB-436 (BRCA1-deficient), Capan-1 (BRCA2-deficient) cells
Concentration: 0.1, 0.3, 1 µM
Incubation Time: 48 h
Result: Showed antiproliferative activity with IC50s of 0.27, 1.43, 0.87, 0.19 µM for UWB1.289, UWB1.289+BRCA1, Capan-1 and MDA-MB-436 cells, respectively.

Immunofluorescence[1]

Cell Line: MDA-MB-436, Capan-1 cells
Concentration: 10 µM
Incubation Time: 48 h
Result: Enhanced the protein levels of phosphorylated Chk1 but the levels of corresponding total proteins were not altered.

Cell Cycle Analysis[1]

Cell Line: Capan-1 cells
Concentration: 1, 3, 10 µM
Incubation Time: 48 h
Result: Induced G2/M arrest in BRCA-deficient cells in a concentration-dependent manner.

Apoptosis Analysis[1]

Cell Line: MDA-MB-436 cells
Concentration: 1, 5, 10 µM
Incubation Time: 96 h
Result: Caused apoptosis in a concentration-dependent manner in MDA-MB-436 cells.

Western Blot Analysis[1]

Cell Line: MDA-MB-436, Capan-1 cells
Concentration: 0.1, 0.3, 1 µM
Incubation Time: 48 h
Result: Induced increased levels of γH2AX in a concentration-dependent manner in both MDA-MB-436 and Capan-1 cells.
分子量

741.93

Formula

C43H56FN5O5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

PARP1-IN-12 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PARP1-IN-12PARPApoptosispoly ADP ribose polymerase γH2AXUWB1.289MDA-MB-436Capan-1BRCADSBsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
PARP1-IN-12
目录号：
HY-150765
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z