Poly-D-lysine hydrobromide (MW 30000-70000) 

Poly-D-lysine hydrobromide (MW 30000-70000) is a synthetic polymeric substrate and is one of the most widely used substrate in neural cell culture. Poly-D-lysine hydrobromide (MW 30000-70000) removes proteinase K-resistant PrP from prion-infected SN56 neuroblastoma cells without affecting PrP^C[1][2].

Poly-D-lysine is immunogenic in rabbits only when injected at low doses^[1].
The approximate concentration of Poly-D-lysine (PDL) needed to lower the amount of PrPres by 50% corresponds to ~0.8 μg/mL or 50 nM^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

30000-70000

(C₆H₁₄N₂O₂)x.xHBr

27964-99-4

油状物

Light brown to brown

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. HansVermeersch, et al. Immunogenicity of poly-D-lysine, a potential polymeric drug carrier. Journal of Controlled Release. Volume 32, Issue 3, 1 December 1994, Pages 225-229.

  [2]. Zhou Xu, et al. Mechanistic insights into cellular alteration of prion by poly-D-lysine: the role of H2H3 domain. FASEB J. 2011 Oct;25(10):3426-35.  [Content Brief]