双分子荧光互补实验 (Bimolecular Fluorescence Complementation, BiFC) 是一种用于研究蛋白相互作用的生物成像技术。通过将目标蛋白的两个部分分别连接到荧光蛋白的两个互补片段，当这两个部分相互结合时，荧光蛋白会重新组装，产生荧光信号。BiFC 可在活体细胞中实时监测蛋白相互作用的时空动态，为细胞信号传导和蛋白互作研究提供了直观、高分辨率的工具。

Bimolecular Fluorescence Complementation (BiFC) is a biological imaging technology used to study protein interactions. By connecting two parts of the target protein to two complementary fragments of a fluorescent protein, when the two parts bind to each other, the fluorescent protein reassembles, producing a fluorescent signal. BiFC can monitor the spatiotemporal dynamics of protein interactions in living cells in real time, providing an intuitive, high-resolution tool for cell signaling and protein interaction research.