巨自噬是一种与常规自噬不同的细胞自噬过程，涉及通过液泡（macropinosome）内含物的融合来清除大型细胞内外的颗粒。检测巨自噬可以使用各种技术，包括显微镜观察巨自噬泡形成、流式细胞术、荧光探针标记以及相关基因或蛋白的表达分析。巨自噬的研究有助于了解这一细胞自噬过程在免疫应答、病原体清除、细胞存活和疾病发生中的功能。

Macroautophagy is an autophagy process that is different from conventional autophagy and involves the clearance of large intracellular and extracellular particles through the fusion of vacuolar (macropinosome) contents. Various techniques can be used to detect macroautophagy, including microscopic observation of macroautophagic vacuole formation, flow cytometry, fluorescent probe labeling, and expression analysis of related genes or proteins. The study of macroautophagy helps to understand the function of this autophagy process in immune response, pathogen clearance, cell survival, and disease development.