多序列比对是将多个生物学序列（例如 DNA、RNA 或蛋白序列）进行比对，以识别它们之间的相似性和差异性。这种比对有助于理解生物学序列的演化关系、功能域的保守性和变异性。多序列比对可以通过多种方法实现，如ClustalW、MAFFT、Muscle等。这在生物信息学、基因组学和系统生物学研究中具有重要作用，为研究基因家族、演化关系和功能域提供了关键的分析工具。

Multiple sequence alignment is the alignment of multiple biological sequences (such as DNA, RNA or protein sequences) to identify similarities and differences between them. This kind of alignment helps to understand the evolutionary relationship of biological sequences, the conservation and variability of functional domains. Multiple sequence alignment can be achieved through a variety of methods, such as ClustalW, MAFFT, Muscle, etc. This plays an important role in bioinformatics, genomics and systems biology research, providing a key analytical tool for studying gene families, evolutionary relationships and functional domains.