Notch 通路检测是一种用于分析和识别 Notch 信号通路活性的实验方法。Notch 通路在细胞分化、增殖和凋亡等过程中发挥关键作用。为了检测 Notch 通路活性，常用的方法包括实时荧光定量 PCR（qPCR）检测 Notch 通路相关基因表达、Western blot 分析 Notch 通路蛋白的翻译水平，以及免疫组化或免疫荧光染色检测 Notch 通路蛋白在细胞或组织中的表达和定位。

Notch pathway assay is an experimental method used to analyze and identify the activity of the Notch signaling pathway. The Notch pathway plays a key role in processes such as cell differentiation, proliferation, and apoptosis. In order to detect Notch pathway activity, commonly used methods include real-time fluorescence quantitative PCR (qPCR) to detect Notch pathway-related gene expression, Western blot to analyze the translation level of Notch pathway proteins, and immunohistochemistry or immunofluorescence staining to detect Notch pathway proteins in cells or tissues. expression and positioning.