酵母双杂交（Yeast Two-Hybrid, Y2H）是一种用于研究蛋白相互作用的分子生物学技术。该方法利用酵母细胞中的转录因子的分离来检测蛋白间的相互作用。一个感应体系中，目标蛋白的两个互补片段与分别与两个不同的蛋白相互作用的部分结合，触发转录因子的重新组装。这种技术对于筛选和验证蛋白-蛋白相互作用在生物学中的重要性非常有用。

Yeast Two-Hybrid (Y2H) is a molecular biology technique used to study protein interactions. This method uses the isolation of transcription factors in yeast cells to detect protein-protein interactions. In a sensing system, two complementary fragments of a target protein bind to parts that interact with two different proteins, triggering the reassembly of the transcription factor. This technique is useful for screening and validating the importance of protein-protein interactions in biology.