14-3-3 beta 蛋白, Cynomolgus

14-3-3 beta proteins act as adapters, binding to phosphoserine or phosphothreonine motifs in different signaling pathways, regulating chaperone activity. It negatively regulates osteogenesis by blocking the nuclear translocation of phosphorylated SRPK2 and counteracting the pro-apoptotic effect of SRPK2 through cyclin D1 expression. 14-3-3 beta Protein, Cynomolgus is the recombinant cynomolgus-derived 14-3-3 beta protein, expressed by E. coli , with tag free. The total length of 14-3-3 beta Protein, Cynomolgus is 244 a.a., with molecular weight of ~28 kDa.

14-3-3 β 蛋白作为接头参与多种信号传导途径的调节，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴结合。由此产生的相互作用通常会调节结合配偶体的活性。作为成骨的负调节因子，它阻碍 SRPK2 磷酸化形式的核转位，抵消 SRPK2 对细胞周期蛋白 D1 表达的刺激作用，从而防止神经元凋亡。此外，14-3-3 beta 负向调节信号级联，通过 AKAP13 激活 MAP 激酶。它以同二聚体形式存在，与多种蛋白质相互作用，包括 SAMSN1、PRKCE、AKAP13、SSH1、TORC2/CRTC2、ABL1、ROR2、GAB2、YAP1、SRPK2、AANAT、MYO1C、SIRT2、DAPK2、PI4KB、TBC1D22A、TBC1D22B、SOS1、 SLITRK1、SYNPO2、RIPOR2、MARK2、MARK3、TESK1、MEFV、HDAC4 和 ADAM22，参与多种细胞过程。

Cynomolgus

E. coli

Tag Free

Q4R572-2 (M1-N244)

101926442  [NCBI]

MDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN

Approximately 28 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.