14-3-3 sigma 蛋白, Mouse

14-3-3 sigma protein, an adapter in diverse signaling pathways, binds phosphoserine or phosphothreonine motifs, modulating partner activity. It regulates protein synthesis and cell growth via Akt/mTOR pathway stimulation when bound to KRT17. It may also regulate MDM2 autoubiquitination, activating p53/TP53. Existing as a homodimer, it interacts with GAB2, KRT17, SAMSN1, SRPK2, COPS6, COP1 (for degradation), phosphorylated DAPK2, PI4KB, SLITRK1, and LRRK2 (phosphorylation-dependent). Complexes include XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, and VPS35. 14-3-3 sigma Protein, Mouse is the recombinant mouse-derived 14-3-3 sigma protein, expressed by E. coli, with tag free. The total length of 14-3-3 sigma Protein, Mouse is 248 a.a., with molecular weight of ~32 kDa.

14-3-3 sigma 蛋白作为衔接子发挥作用，涉及广泛的一般和专门信号传导途径的调节，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴结合，从而调节结合伙伴的活性。特别是，当与 KRT17 结合时，它通过刺激 Akt/mTOR 途径发挥调节蛋白质合成和上皮细胞生长的作用。此外，它还可能调节 MDM2 自动泛素化和降解，从而导致 p53/TP53 的激活。14-3-3 sigma 以同二聚体形式存在，与 XPO7、EIF4A1、ARHGAP1、VPS26A、VPS29 和 VPS35 形成复合物，并与多种蛋白质相互作用，包括 GAB2、KRT17、SAMSN1、SRPK2、COPS6、COP1（导致蛋白酶体降解）、DAPK2、PI4KB、SLITRK1 和 LRRK2 的“Thr-369”磷酸化形式（依赖于 LRRK2 磷酸化）。这些不同的相互作用凸显了 14-3-3 sigma 在协调各种细胞过程和信号事件中的多功能作用。

Mouse

E. coli

Tag Free

O70456 (M1-S248)

55948  [NCBI]

MERASLIQKAKLAEQAERYEDMAAFMKSAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVKEYREKVETELRGVCDTVLGLLDSHLIKGAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADSAGEEGGEAPEEPQS

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.