Aconitase 1/ACO1 蛋白, Human (sf9, His)

The ACO1 protein is a bifunctional iron sensor that dynamically adapts to cellular iron levels. Under conditions of iron deficiency, it is converted to mRNA binding and regulates iron-related gene expression. Aconitase 1/ACO1 Protein, Human (sf9, His) is the recombinant human-derived Aconitase 1/ACO1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Aconitase 1/ACO1 Protein, Human (sf9, His) is 889 a.a., with molecular weight of ~90 kDa.

乌头酸酶 1/ACO1 蛋白作为双功能铁传感器，根据细胞铁可用性动态调整其活性。在铁缺乏的条件下，它会转变为 mRNA 结合活性，调节涉及铁吸收、隔离和利用的基因的表达。ACO1 与目标 mRNA 非翻译区域的铁反应元件 (IRES) 结合，从而调节翻译，例如，抑制铁蛋白和氨基乙酰丙酸合酶的翻译，同时稳定转铁蛋白受体的 mRNA。相反，当细胞铁水平升高时，ACO1 形成 4Fe-4S 簇，阻止 RNA 结合活性并促进其乌头酸酶活性。在这种模式下，ACO1 通过顺乌头酸催化柠檬酸异构化为异柠檬酸。这种双重功能强调了 ACO1 在控制细胞对不同铁浓度反应的复杂调节网络中的关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P21399 (M1-K889)

48  [NCBI]

Approximately 90 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.