1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ACPL2 蛋白, Human (HEK293, His)

ACPL2 蛋白, Human (HEK293, His)

目录号: HY-P76713
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ACPL2 蛋白通过催化蛋白聚糖中木糖的 2-O-去磷酸化,在糖胺聚糖 (GAG) 链的调节中发挥关键作用。此步骤对于硫酸化 GAG(包括硫酸乙酰肝素和硫酸软骨素)的合成至关重要。ACPL2 蛋白, Human (HEK293, His) 是重组的 ACPL2 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

The ACPL2 protein plays a critical role in the regulation of glycosaminoglycan (GAG) chains by catalyzing the 2-O-dephosphorylation of xylose in proteoglycans. This step is critical for the synthesis of sulfated GAGs, including heparan sulfate and chondroitin sulfate. ACPL2 Protein, Human (HEK293, His) is the recombinant human-derived ACPL2 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

ACPL2 蛋白通过催化蛋白聚糖糖胺聚糖-蛋白质连接区域内木糖的 2-O-去磷酸化,在成熟糖胺聚糖 (GAG) 链的调节中发挥着至关重要的作用。该过程对于在核心蛋白的共同GAG-蛋白连接区(GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser)上合成硫酸化GAG(例如硫酸乙酰肝素和硫酸软骨素)具有重要意义。通过特定糖基转移酶逐步添加单糖残基导致该连接区域的形成,其中木糖 2-O-去磷酸化是 GAG 链重复二糖区域起始和有效延伸的先决条件。ACPL2 在此背景下的酶活性强调了其在 GAG 链组装的微调控制中的关键作用,有助于糖胺聚糖结构的精确调节。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

Q8TE99-1/NP_689495.1 (M1-F480)

基因 ID

92370  [NCBI]

蛋白结构
N-term
ACPL2 (M1-F480)
Accession # Q8TE99-1/NP_689495.1
His
C-term
同用名
2-phosphoxylose phosphatase 1; Acid phosphatase-like protein 2; PXYLP1; HEL124
分子量

Approximately 50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ACPL2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ACPL2 Protein, Human (HEK293, His)
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