1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ACYP1 蛋白, Human (His)

ACYP1 蛋白, Human (His)

目录号: HY-P7458
产品使用指南

ACYP1 蛋白, Human (His)是带有 N 末端 His 标签的人重组 Acylphosphatase-1 (ACYP1),在大肠杆菌中表达。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

ACYP1 Protein, Human (His) is human recombinant Acylphosphatase-1 (ACYP1) with an N-terminal His tag. Recombinant Human Acylphosphatase-1, His is expressed in E. coli.

研究背景

酰基磷酸酶-1 (ACYP1) 是一种小型胞质酶,可催化酰基磷酸酯的羧基磷酸键的水解。酰基磷酸酶以两种同工酶形式广泛分布于人体中,称为ACYP1和ACYP2。人类ACYP1基因全长约10Kbp,包含84bp和123bp两个外显子。存在经典的内含子-外显子连接信号序列和基因末端的聚腺苷酸化位点[1]

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P07311 (M1-K99)

基因 ID

97  [NCBI]

蛋白结构
N-term
6*His
ACYP1 (M1-K99)
Accession # P07311
C-term
中文名
重组人酰基磷酸酶-1,His
同用名
rHuAcylphosphatase-1, His; ACYP-1; Acylphosphatase 1
氨基酸序列

HHHHHHMAEGNTLISVDYEIFGKVQGVFFRKHTQAEGKKLGLVGWVQNTDRGTVQGQLQGPISKVRHMQEWLETRGSPKSHIDKANFNNEKVILKLDYSDFQIVK

分子量

Approximately 14.0 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

ACYP1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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