ADAMTS13 蛋白, Human (CHO, His)

The ADAMTS13 protein, a metalloproteinase, has a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 motif. It cleaves von Willebrand Factor and defects in this gene cause thrombotic thrombocytopenic purpura. Alternative splicing generates multiple transcript variants. ADAMTS13 is broadly expressed in various tissues, including the liver, testis, and 24 other tissues. ADAMTS13 Protein, Human (CHO, His) is the recombinant human-derived ADAMTS13, expressed by CHO , with C-10*His labeled tag. The total length of ADAMTS13 Protein, Human (CHO, His) is 655 a.a.,

ADAMTS13 蛋白是金属蛋白酶家族的成员，包含不同的区域，包括金属蛋白酶结构域、解整合素样结构域和 1 型血小板反应蛋白 (TS) 基序。这种酶特异性裂解血管性血友病因子 (vWF)，该基因的缺陷与血栓性血小板减少性紫癜有关。通过选择性剪接产生多个转录变体。此外，ADAMTS13 蛋白在多种组织中广泛表达，包括肝脏、睾丸和 24 种其他组织。

Measured by its ability to cleave 4µM fluorogenic peptide substrate, FRETS-VWF73 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 595.51 pmol/min/µg.

Human

CHO

C-10*His

NP_620594 (Q34-W688)

11093  [NCBI]

QQSCLQALEPQAVSSYLSPGAPLKGRPPSPGFQRQRQRQRRAAGGILHLELLVAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPSLGAQFRVHLVKMVILTEPEGAPNITANLTSSLLSVCGWSQTINPEDDTDPGHADLVLYITRFDLELPDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLGVTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASDGAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDPPRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKAVACTFAREHLDMCQALSCHTDPLDQSSCSRLLVPLLDGTECGVEKWCSKGRCRSLVELTPIAAVHGRWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFGGRACVGADLQAEMCNTQACEKTQLEFMSQQCARTDGQPLRSSPGGASFYHWGAAVPHSQGDALCRHMCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTLSLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDNSTCSPRKGSFTAGRAREYVTFLTVTPNLTSVYIANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPSLLEDGRVEYRVALTEDRLPRLEEIRIWGPLQEDADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQAWVW

Approximately 90 kDa due to the glycosylation.

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.