1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Adenosine Deaminase/ADA 蛋白, Human (sf9, His)

Adenosine Deaminase/ADA 蛋白, Human (sf9, His)

目录号: HY-P74429
产品使用指南

腺苷脱氨酶/ADA 蛋白催化腺苷和 2-脱氧腺苷的水解脱氨基,这对于嘌呤代谢和腺苷稳态至关重要。它通过调节细胞外腺苷间接影响细胞信号传导。Adenosine Deaminase/ADA 蛋白, Human (sf9, His) 是重组的 腺苷脱氨酶/ADA 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。Adenosine Deaminase/ADA 蛋白, Human (sf9, His) 全长 363 个氨基酸,分子量约为 ~44 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The adenosine deaminase/ADA protein catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine, which is critical for purine metabolism and adenosine homeostasis. It affects cell signaling indirectly by regulating extracellular adenosine. Adenosine Deaminase/ADA Protein, Human (sf9, His) is the recombinant human-derived Adenosine Deaminase/ADA protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Adenosine Deaminase/ADA Protein, Human (sf9, His) is 363 a.a., with molecular weight of ~44 kDa.

研究背景

腺苷脱氨酶 (ADA) 蛋白是一种关键酶,负责协调腺苷和 2-脱氧腺苷的水解脱氨基作用。这种酶活性是嘌呤代谢的基础,并且对腺苷稳态有显着贡献。除了在核苷酸分解代谢中的直接作用之外,ADA 在细胞信号转导事件中发挥着多方面的作用。它作为 T 细胞共激活的正调节因子,通过与 DPP4 相互作用影响淋巴细胞-上皮细胞粘附。此外,ADA 还能增强树突状细胞的免疫原性,影响共刺激分子的表达和细胞因子的分泌。该蛋白还调节腺苷受体 ADORA1 和 ADORA2A,通过构象变化促进配体亲和力。此外,ADA 参与纤溶酶原激活,影响 CD4+ T 细胞分化和增殖,并在男性生育力和早期植入后胚胎发育中发挥作用。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P00813 (M1-L363)

基因 ID

100  [NCBI]

蛋白结构
N-term
ADA (M1-L363)
Accession # P00813
His
C-term
同用名
Adenosine deaminase; ADA; ADA1
分子量

Approximately 44 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Adenosine Deaminase/ADA Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Adenosine Deaminase/ADA Protein, Human (sf9, His)
目录号:
HY-P74429
需求量: