2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ADH1A 蛋白, Human (His)

ADH1A 蛋白, Human (His)

目录号: HY-P701925
产品使用指南

ADH1A 蛋白被认为是乙醇脱氢酶，在细胞代谢中至关重要。它催化伯醇和仲醇的氧化。尽管乙醇是一种较差的底物，但 ADH1A 的广泛特异性强调了其在氧化多种醇、维持细胞氧化还原平衡方面的重要性。该酶的多功能性凸显了其在酒精相关代谢途径中的关键作用。ADH1A 蛋白, Human (His) 是重组的 ADH1A 蛋白，由 E. coli 表达，带有 N-6*His 标签。ADH1A 蛋白, Human (His) 全长 375 个氨基酸。

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描述

ADH1A Protein, recognized as alcohol dehydrogenase, is pivotal in cellular metabolism. It catalyzes the oxidation of both primary and secondary alcohols. Despite ethanol being a poor substrate, ADH1A's broad specificity underscores its importance in oxidizing diverse alcohols, maintaining cellular redox balance. The enzyme's versatility highlights its crucial role in alcohol-related metabolic pathways. ADH1A Protein, Human (His) is the recombinant human-derived ADH1A protein, expressed by E. coli , with N-6*His labeled tag. The total length of ADH1A Protein, Human (His) is 375 a.a., .
研究背景

ADH1A 被称为乙醇脱氢酶，通过催化伯醇和仲醇的氧化在细胞代谢中发挥关键作用。尽管乙醇是 ADH1A 的底物，但该酶更广泛的底物特异性强调了其在细胞过程中多种醇氧化中的重要性。这种催化功能有助于维持细胞氧化还原平衡，并凸显了 ADH1A 在酒精相关代谢途径中的多功能性。
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

P07327 (M1-F375)
基因 ID

124
蛋白结构
N-term
6*His
ADH1A (M1-F375)
Accession # P07327
C-term
同用名
ADH1A; Alcohol dehydrogenase 1A; Alcohol dehydrogenase subunit alpha
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ADH1A Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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