1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. adhT 蛋白, Geobacillus stearothermophilus

adhT 蛋白, Geobacillus stearothermophilus

目录号: HY-P702140
产品使用指南

adhT 是一种 NAD(+) 依赖性乙醇脱氢酶,催化醇的氧化,将其转化为醛或酮,同时将 NAD(+) 还原为 NADH。其特异性能够参与多种代谢途径,有助于酒精分解和 NADH 再生。adhT 是酒精代谢不可或缺的一部分,可维持细胞氧化还原平衡和能量稳态,这对于发酵和能量生产等过程至关重要。adhT 蛋白, Geobacillus stearothermophilus 是重组的 adhT 蛋白,由 E. coli 表达,不带标签。adhT 蛋白, Geobacillus stearothermophilus 全长 336 个氨基酸。

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描述

adhT, a NAD(+)-dependent alcohol dehydrogenase, catalyzes the oxidation of alcohols, converting them to aldehydes or ketones while reducing NAD(+) to NADH. Its specificity enables participation in diverse metabolic pathways, contributing to alcohol breakdown and NADH regeneration. Integral to alcohol metabolism, adhT maintains cellular redox balance and energy homeostasis, crucial for processes like fermentation and energy production. adhT Protein, Geobacillus stearothermophilus is the recombinant adhT protein, expressed by E. coli , with tag free. The total length of adhT Protein, Geobacillus stearothermophilus is 336 a.a., .

研究背景

adhT 是一种 NAD(+) 依赖性乙醇脱氢酶,是一种使用 NAD(+) 作为辅助因子催化醇氧化的蛋白质。这种酶活性涉及将醇转化为相应的醛或酮,同时将 NAD(+) 还原为 NADH。adhT 的特异性使其能够参与各种代谢途径,有助于酒精的分解和 NADH 的再生。作为酒精代谢的关键参与者,adhT 在维持细胞氧化还原平衡和能量稳态方面发挥着重要作用。它的活性是发酵等过程不可或缺的一部分,在发酵等过程中,酒精的转化是能源生产的关键步骤。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

P12311 (K2-D337)

基因 ID

/

蛋白结构
N-term
adhT (K2-D337)
Accession # P12311
C-term
同用名
adhT; Alcohol dehydrogenase; ADH-T
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

adhT Protein, Geobacillus stearothermophilus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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adhT Protein, Geobacillus stearothermophilus
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HY-P702140
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