1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ADI 蛋白, Metamycoplasma hominis (HEK293, His)

ADI 蛋白, Metamycoplasma hominis (HEK293, His)

目录号: HY-P700625
COA 产品使用指南

ADI 蛋白是 ADI 途径的核心,催化 L-精氨酸降解的第一步。具体来说,它将 L-精氨酸转化为氨基甲酰磷酸,启动该途径并在调节 L-精氨酸的整体代谢分解中发挥关键作用。ADI 蛋白, Metamycoplasma hominis (HEK293, His) 是重组的 ADI 蛋白,由 HEK293 表达,带有 C-10*His 标签。ADI 蛋白, Metamycoplasma hominis (HEK293, His) 全长 408 个氨基酸,分子量为 50 kDa。

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规格 价格 是否有货 数量
20 μg ¥3600 In-stock
50 μg ¥6800 In-stock
100 μg ¥11000 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ADI Protein, Metamycoplasma hominis (HEK293, His) is 408 a.a., with molecular weight of 50 kDa.

研究背景

ADI 是通过 ADI 途径降解 L-精氨酸的核心酶,催化该氨基酸降解过程的第一步。具体来说,ADI 在标志着该途径开始的关键酶促反应中促进 L-精氨酸转化为氨基甲酰磷酸。此步骤对于 L-精氨酸的整体代谢分解至关重要,展示了 ADI 在调节该氨基酸降解途径中的关键作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Others

表达系统

HEK293

标签

C-10*His

蛋白编号

P41141 (S2-W409)

基因 ID

/

蛋白结构
N-term
ADI (S2-W409)
Accession # P41141
10*His
C-term
同用名
Arginine deiminase; Arginine dihydrolase (AD); arcA
氨基酸序列

SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW

分子量

50kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ADI Protein, Metamycoplasma hominis (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ADI Protein, Metamycoplasma hominis (HEK293, His)
目录号:
HY-P700625
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