Agrin 蛋白, Human (CHO, His)

Agrin is critical for neuromuscular junction development and was discovered in mouse knockout studies. It contains laminin G, Kazal-type serpin, and epidermal growth factor domains. Agrin Protein, Human (CHO, His) is the recombinant human-derived Agrin protein, expressed by CHO , with N-6*His labeled tag.

Agrin 蛋白是神经肌肉接头 (NMJ) 发育的重要组成部分，已通过小鼠敲除研究得到鉴定。它包含层粘连蛋白 G、Kazal 型丝氨酸蛋白酶抑制剂和表皮生长因子结构域。此外，还会发生翻译后修饰，包括添加糖胺聚糖和二硫键。值得注意的是，该基因的突变与影响特定家族的肢带肌肉的先天性肌无力综合征有关。选择性剪接产生编码不同亚型的多种转录变体。该蛋白表现出广泛的表达模式，在肾、肺和 21 种其他组织中显着存在。

Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells. The ED₅₀ this effect is <113.4 ng/mL after 1 hour incubation at 37 °C, corresponding to a specific activity is > 8.8183×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The ED₅₀ for this effect is 113.4 ng/ml after 1 hour incubation at 37 °C, corresponding to a specific activity is > 8.8183×10³ units/mg.

Human

CHO

N-6*His

O00468-6/NP_940978 (A1260-P2045)

375790  [NCBI]

ATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

97.12 kDa-110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 300 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.