1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. AGRP 蛋白, Mouse (HEK293, Fc)

AGRP 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75525
产品使用指南

AGRP 蛋白对于体重稳态至关重要,并通过中枢黑皮质素系统调节进食行为。作为 α 黑素细胞刺激激素的拮抗剂,AGRP 通过下丘脑和肾上腺中的黑皮质素受体抑制 cAMP 的产生。AGRP 蛋白, Mouse (HEK293, Fc) 是重组的 AGRP 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

The AGRP protein is critical for body weight homeostasis and regulates feeding behavior through the central melanocortin system. As an antagonist of alpha melanocyte-stimulating hormone, AGRP inhibits cAMP production through melanocortin receptors in the hypothalamus and adrenal glands. AGRP Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived AGRP protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

AGRP 蛋白在维持体重稳态中起着至关重要的作用。它通过中枢黑皮质素系统参与进食行为的调节。AGRP 通过抑制刺激下丘脑和肾上腺黑皮质素受体介导的 cAMP 产生,充当 α 黑素细胞刺激激素的拮抗剂。它对 MC5R 表现出最小的活性,但作为 MC3RMC4R 的反向激动剂发挥作用,有效抑制它们的组成型活性。AGRP 还以抑制蛋白依赖性方式促进 MC3RMC4R 的内吞作用。此外,它与黑皮质素受体 MC3R、MC4R 和 MC5R 相互作用。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P56473 (M1-T131)

基因 ID
蛋白结构
N-term
AGRP (M1-T131)
Accession # P56473
hFc
C-term
同用名
Agouti-related protein; AGRP; AGRT; ART
分子量

Approximately 41 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AGRP Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AGRP Protein, Mouse (HEK293, Fc)
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