1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. AK3 蛋白, Human (Myc, His)

AK3 蛋白, Human (Myc, His) 是一种线粒体蛋白,位于线粒体基质中。

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描述

AK3 Protein, Human (Myc, His) is a mitochondrial protein and is localized in the mitochondrial matrix.

研究背景

腺苷酸激酶(以下简称AK)催化腺嘌呤核苷酸之间的可逆高能磷酰基转移反应。迄今为止,已鉴定出六种 AK 同工酶:AK1、AK2、AK3、AK4、AK5 和 AK6。AK3在除红细胞外的所有组织中均有表达,表明AK3基因是看家型基因。AK3 催化 1 GDP 或 ADP 分子形成或使用 GTP(而不是 ATP)作为磷酸盐捐赠底物的逆反应。该AK3分别利用底物水平的柠檬酸循环中磷酸化产生的GTP和基质中存在的AMP生成GDP和ADP,并且生成的GDP被用于柠檬酸循环的下一个循环。而 ADP 则用作线粒体 ATP 合成酶的底物[1]

种属

Human

表达系统

E. coli

标签

N-His;C-Myc

蛋白编号

Q9UIJ7-1 (M1-P227)

基因 ID
蛋白结构
N-term
10*His
AK3 (M1-P227)
Accession # Q9UIJ7-1
C-term
同用名
Adenylate kinase 3 alpha-like 1; Adenylate kinase 3; Adenylate kinase 3, formerly; adenylate kinase 6, adenylate kinase 3 like 1; AK 3; mitochondrial
氨基酸序列

MGASARLLRAVIMGAPGSGKGTVSSRITTHFELKHLSSGDLLRDNMLRGTEIGVLAKAFIDQGKLIPDDVMTRLALHELKNLTQYSWLLDGFPRTLPQAEALDRAYQIDTVINLNVPFEVIKQRLTARWIHPASGRVYNIEFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEDQTKPVLEYYQKKGVLETFSGTETNKIWPYVYAFLQTKVPQRSQKASVTP

分子量

Approximately 32.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

AK3 Protein, Human (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AK3 Protein, Human (Myc, His)
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HY-P71645
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