2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. aKMT 蛋白, Sulfolobus islandicus (His, Strep)

aKMT 蛋白, Sulfolobus islandicus (His, Strep)

目录号: HY-P701941
产品使用指南

赖氨酸甲基转移酶 (aKMT) 负责催化靶蛋白中赖氨酸残基的甲基化，使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体。该酶表现出广泛的底物特异性，在体外表现出主要在穹窿染色质蛋白 Cren7 的“Lys-11”、“Lys-16”和“Lys-31”处以及几种重组硫化叶菌蛋白的甲基化能力。aKMT 蛋白, Sulfolobus islandicus (His, Strep) 是重组的 aKMT 蛋白，由 E. coli 表达，带有 N-Strep, N-6*His 标签。aKMT 蛋白, Sulfolobus islandicus (His, Strep) 全长 160 个氨基酸。

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描述

Lysine methyltransferase (aKMT) is responsible for catalyzing the methylation of lysine residues in target proteins, using S-adenosyl-L-methionine (SAM) as a methyl donor. The enzyme exhibits broad substrate specificity, showing methylation primarily at "Lys-11", "Lys-16" and "Lys-31" of the vault chromatin protein Cren7 as well as several recombinant Sulfolobus proteins in vitro Ability. aKMT Protein, Sulfolobus islandicus (His, Strep) is the recombinant aKMT protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of aKMT Protein, Sulfolobus islandicus (His, Strep) is 160 a.a., .
研究背景

aKMT 蛋白充当赖氨酸甲基转移酶，催化靶蛋白中赖氨酸残基的甲基化。这种酶活性是使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体来完成的。aKMT 显示出广泛的底物特异性，并且能够甲基化各种靶蛋白。特别是，已观察到在特定的赖氨酸残基（包括“Lys-11”、“Lys-16”和“Lys-31”）处甲基化了穹窿染色质蛋白 Cren7。此外，aKMT 表现出序列无关的赖氨酸甲基化，展示了其在体外修饰一系列靶蛋白方面的多功能性。
种属

Others
表达系统

E. coli
标签

N-Strep;N-6*His
蛋白编号

F0NBH8 (S2-K161)
基因 ID

8761611
蛋白结构
N-term
Strep-6*His
aKMT (S2-K161)
Accession # F0NBH8
C-term
同用名
Protein-lysine N-methyltransferase; Archaeal protein lysine methyltransferase; aKMT
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

aKMT Protein, Sulfolobus islandicus (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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